APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY

流式细胞术在细胞生物学中的应用

• 批准号: 3774387
• 负责人: S O SHARROW
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774387
• 关键词: T lymphocyte; antigen presentation; biological signal transduction; biomedical resource; bone marrow transplantation; cell adhesion molecules; cell differentiation; flow cytometry; graft versus host disease; health science research support; histocompatibility antigens; single cell analysis; transplant rejection

The Experimental Immunology Branch flow cytometry laboratory currently supports multiple research projects for more than 40 investigators. These investigations involve quantitative single cell analysis of parameters associated with cells freshly prepared from different species/tissues, as well as a spectrum of in vitro cultured cells. Basic research support is provided to members of the EIB as well as to other investigators within DCBDC. Currently supported projects include, but are not limited to, the following areas of study: a) in vivo and in vitro analyses of intra- cellular signalling via T cell surface molecules, b) analyses of cellular defects in animals with genetic or induced immune dysfunction; c) studies of the pathogenesis of graft-versus-host disease; d) analyses of the coordinate cell surface expression of cell adhesion molecules; e) investigations of T cell ontogeny and differentiation; f) studies of mechanisms of T cell repertoire generation; g) analyses of expression of transplantation antigens; h) investigations of mechanisms involved in antigen presentation processes; and i) analyses of the mechanisms involved in marrow graft rejection versus acceptance.

实验免疫学分支流式细胞术实验室目前 支持40多名研究人员的多个研究项目。 这些 研究涉及参数的定量单细胞分析 与从不同种属/组织新鲜制备的细胞相关，如 以及体外培养细胞的光谱。 基础研究支持是 提供给EIB成员以及其他调查人员， DCBDC。目前支持的项目包括但不限于 以下研究领域：a）在体内和体外分析内， 通过T细胞表面分子的细胞信号传导，B）细胞信号传导的分析， 具有遗传性或诱导性免疫功能障碍的动物的缺陷; c）研究 移植物抗宿主病的发病机制; d）分析 协调细胞粘附分子的细胞表面表达; e） T细胞个体发育和分化的研究; f） T细胞库产生的机制; g）分析T细胞库的表达; 移植抗原; h）研究参与移植的机制， 抗原呈递过程;和i）分析所涉及的机制 骨髓移植排斥与接受的对比