ANTIBODIES RECOGNIZING SPECIFIC MUCOUS GLYCOPROTEINS FROM HUMAN RESPIRATORY CELLS

气道分泌物过多是阻塞性 以气道炎症为特征的气道疾病。呼吸 粘液糖蛋白（MGP）由至少两种细胞类型产生， 呼吸道至少有四种粘蛋白基因表达于 气道上皮这些糖蛋白相对 在大小上不均匀;然而， 可用于不同的MGP基因产物。因此，定量 每一种粘蛋白基因产物的分离是很困难的。 用于产生融合蛋白的表达质粒， 含有非抗原性蛋白质和来自每一种的独特表位。 已知的产品正在开发中 用于测量特定粘蛋白的酶联免疫吸附测定 培养基和气道分泌物中的基因产物。

Excessive airway secretions are a prominent feature of obstructive airway diseases characterized by airway inflammation. Respiratory mucus glycoproteins (MGP) are produced by at least two cell types in the respiratory tract. At least four mucin genes are expressed in the airway epithelium. These glycoproteins are relatively heterogenous in size; however, no specific characteristics are available for different MGP gene products. Therefore, quantitation of each specific mucin gene product has been difficult. Expression plasmids for the production of fusion proteins that contain a nonantigenic protein and a unique epitope from each of the known products are being developed for the purpose of developing enzyme-linked immunoabsorbent assays for measuring specific mucin gene products in culture media and in airway secretions.