INVESTIGATION OF LYMPHOCYTE PHENOTYPE USING DUAL COLOR FLOW CYTOMETRY

正常外周血淋巴细胞正在使用双 彩色流式细胞术评估淋巴细胞表型。该研究还 比较两种细胞制备方法。这项研究已被扩展 在正常个体中包括三色流式细胞术。 额外表面抗原和新淋巴细胞的评估 基于三种表面抗原评估的亚群 同时正在进行中。另外，方法比较 对于红细胞裂解已确定该技术可以具有 对荧光强度和淋巴细胞恢复的影响。扩展 这些研究以 CD34+ 造血祖细胞鉴定 在这种情况下红细胞裂解的效果与 淋巴细胞免疫表型分析的影响。

Normal peripheral blood lymphocytes are being evaluated using dual- color flow cytometry to assess lymphocyte phenotypes. The study also compares two cell preparation methods. This study has been extended to include three-color flow cytometry in normal individuals. Evaluation of additional surface antigens and new lymphocyte subpopulations based on the assessment of three surface antigens simultaneously is in progress. In addition, a comparison of methods for red cell lysis has established that this technique can have an impact on fluorescence intensity and lymphocyte recovery. Extension of these studies to CD34+ hematopoietic progenitor cells identified differences in the effects of red cell lysis in this setting versus the effects of lymphocyte immunophenotyping.