INVESTIGATION OF LYMPHOCYTE PHENOTYPE USING DUAL COLOR FLOW CYTOMETRY

使用双色流式细胞术研究淋巴细胞表型

• 批准号: 2571414
• 负责人: T A FLEISHER
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2571414
• 关键词: CD antigens; clinical research; erythrocytes; flow cytometry; hematopoietic stem cells; human subject; leukocyte antigen typing; lymphocyte; phenotype

Normal peripheral blood lymphocytes are being evaluated using dual- color flow cytometry to assess lymphocyte phenotypes. The study also compares two cell preparation methods. This study has been extended to include three-color flow cytometry in normal individuals. Evaluation of additional surface antigens and new lymphocyte subpopulations based on the assessment of three surface antigens simultaneously is in progress. In addition, a comparison of methods for red cell lysis has established that this technique can have an impact on fluorescence intensity and lymphocyte recovery. Extension of these studies to CD34+ hematopoietic progenitor cells identified differences in the effects of red cell lysis in this setting versus the effects of lymphocyte immunophenotyping.

正常外周血淋巴细胞用双-测定