INVESTIGATION OF LYMPHOCYTE PHENOTYPE USING DUAL COLOR FLOW CYTOMETRY

正常的外周血淋巴细胞正在用双重- 彩色流式细胞术检测淋巴细胞表型。这项研究还 比较了两种细胞制备方法。这项研究已经扩展了 将三色流式细胞术纳入正常个体。 对附加表面抗原和新淋巴细胞的评价 基于三种表面抗原评估的亚群 同时正在进行中。此外，还对各种方法进行了比较 因为红细胞裂解技术已经确定这种技术可以有一个 对荧光强度和淋巴细胞恢复的影响。延拓 其中CD34造血祖细胞被鉴定为 在这种情况下红细胞溶解的效果与在这种情况下的差异 淋巴细胞免疫表型的影响。

Normal peripheral blood lymphocytes are being evaluated using dual- color flow cytometry to assess lymphocyte phenotypes. The study also compares two cell preparation methods. This study has been extended to include three-color flow cytometry in normal individuals. Evaluation of additional surface antigens and new lymphocyte subpopulations based on the assessment of three surface antigens simultaneously is in progress. In addition, a comparison of methods for red cell lysis has established that this technique can have an impact on fluorescence intensity and lymphocyte recovery. Extension of these studies to CD34+ hematopoietic progenitor cells identified differences in the effects of red cell lysis in this setting versus the effects of lymphocyte immunophenotyping.