ASSESSMENT OF HYDROGEN PEROXIDE GENERATION IN NEUTROPHILS BY FLOW CYTOMETRY

通过流式细胞术评估中性粒细胞中过氧化氢的生成

• 批准号: 2571424
• 负责人: T A FLEISHER
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2571424
• 关键词: chronic granulomatous disease; clinical research; flow cytometry; human subject; hydrogen peroxide; leukocyte oxidative burst; method development; neutrophil

An assay for evaluating hydrogen peroxide production by human neutrophils after stimulation using flow cytometry was developed and applied to patients with chronic granulomatous disease (CGD) and the X-linked obligate carriers. The flow cytometric dihydrorhodamine (DHR)-based assay for evaluation of oxidative burst is a sensitive method for evaluating phagocytes in patients with possible CGD. This assay also appears to be a good screen for the genotype of the disease. In addition, the method was optimized and is sensitive to 1 normal cell in <=10,000 abnormal cells making it an ideal assay for monitoring CGD patients after gene therapy, as well as for identifying normal cells after allogeneic granulocyte transfusion.

一种评价人体产生过氧化氢的方法 用流式细胞术检测刺激后的中性粒细胞 适用于慢性肉芽肿性疾病(CGD)和 X连锁专职携带者。流式细胞术检测双氢罗丹明 以DHR为基础的氧化猝发检测是一种敏感的方法 评估疑似CGD患者吞噬细胞的方法。这 化验似乎也是一种很好的基因筛查方法 疾病。此外，对该方法进行了优化，并对该方法进行了灵敏度分析。 1个正常细胞=10,000个异常细胞使其成为一种理想的检测方法 用于监测基因治疗后的CGD患者，以及 异基因粒细胞输注后对正常细胞的鉴定。