EFFECTS OF FOREIGN GENE EXPRESSION IN THE CNS ON AMYGDA-KINDLED RATS

该项目的目标是用腺病毒载体将基因送入大鼠的大脑。 联合病毒载体抑制杏仁核癫痫发作的实验研究 点燃火种。已经证明AAV是一种有效的基因递送系统 在中枢神经系统中，Bax mRNA的增加可能在细胞丢失中起重要作用 在癫痫发作后，进一步的实验将在 接下来的两步。首先，我们将把Bax反义基因重组到 腺相关病毒载体观察Bax反义寡核苷酸能否阻断细胞死亡或凋亡 培养的细胞。第二个主要步骤将是提供Bax反义 进入点燃的大鼠的海马区，看看它是否能阻止点燃的 癫痫发作。 AAV基因递送系统可用于转移任何基因，其中 我们可能感兴趣(例如，生长因子、CCK、BAX等)进入 老鼠的大脑。这可能对神经降压素的基因治疗具有潜在的价值。 更生性或精神障碍，并将进一步神经科学研究。

The goal of this project is to deliver genes into rat brain using adeno- associated virus (AAV) vector to quench seizures induced by amygdala kindling. Having shown that the AAV is an efficient gene delivery system in the CNS, and that increased Bax mRNA may be important in the cell loss of hippocampus after seizures, further experiments will be done in the next two steps. First, we will recombine the Bax antisense gene into the AAV vector to see if Bax antisense can block cell death or apoptosis in cultured cells. A second major step will be to deliver Bax antisense into the hippocampus of kindled rats to see if it can prevent the kindled seizures. The AAV gene delivery system can be used to transfer any genes in which we might be interested (i.e., growth factors, CCK, Bax, etc.) into the rat brain. This may have potential value for gene therapy of neurodeg- enerative or mental disorders, and will further neuroscience research.