EFFECTS OF FOREIGN GENE EXPRESSION IN THE CNS ON AMYGDA-KINDLED RATS

该项目的目标是使用腺病毒将基因导入大鼠大脑， 相关病毒（AAV）载体抑制杏仁核诱导的癫痫发作 引火柴 已经表明AAV是一种有效的基因递送系统， 在CNS中，Bax mRNA的增加可能是重要的细胞损失 癫痫发作后海马的变化，进一步的实验将在 接下来的两个步骤。 首先，我们将Bax反义基因重组到 AAV载体，以观察Bax反义是否可以阻断细胞死亡或凋亡。 培养细胞 第二个主要步骤是将Bax反义核酸 注入点燃的老鼠的海马体，看看它是否能阻止点燃的 癫痫发作 我们可以使用AAV基因传递系统转移任何感兴趣的基因 (i.e.,生长因子、CCK、Bcl-2等）植入老鼠的大脑 这可能 对神经退行性疾病或精神疾病的基因治疗具有潜在价值 疾病，并将进一步神经科学研究。如果AAV载体具有 在中枢神经系统中的高和长期表达，我们可以使用这个系统， 递送我们感兴趣的基因（即，CCK或生长因子）注射到大鼠体内 个脑袋 这可能对肿瘤的基因治疗具有潜在的应用价值。 神经退行性疾病或精神疾病，并将进一步促进神经科学 research.

The goal of this project is to deliver genes into rat brain using adeno- associated virus (AAV) vector to quench seizures induced by amygdala kindling. Having shown that the AAV is an efficient gene delivery system in the CNS, and that increased Bax mRNA may be important in the cell loss of hippocampus after seizures, further experiments will be done in the next two steps. First, we will recombine the Bax antisense gene into the AAV vector to see if Bax antisense can block cell death or apoptosis in cultured cells. A second major step will be to deliver Bax antisense into the hippocampus of kindled rats to see if it can prevent the kindled seizures. We can use the AAV gene delivery system to transfer any gene of interest (i.e., growth factors, CCK, Bcl-2, etc.) into the rat brain. This may have potential value for gene therapy of neurodegenerative or mental disorders, and will further neuroscience research. If the AAV vector has high and long-term expression in the CNS, we can use this system to deliver our genes of interest (i.e., CCK or growth factor) into the rat brain. This may have potential value for gene therapy of neurodegenerative or mental disorders, and will further neuroscience research.