BINDING FORCES IN RECEPTOR MEDIATED CELL ADHESION

受体介导的细胞粘附中的结合力

• 批准号: 2590321
• 负责人: A EIDSATH
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2590321
• 关键词: cell adhesion; cell adhesion molecules; chemical binding; fluid flow; human immunodeficiency virus; protein structure function; receptor binding; synthetic protein; tissue /cell culture; virus protein; virus receptors

Since it is known that the tat protein of the HIV will enter the cell in vitro and stimulate the production of HIV products, there is almost certainly a receptor site for it on the cell membrane. Segments of this 86 amino acid protein are being synthesized to determine which segment is responsible for binding to the receptor. This phenomenon will be evaluated by quantitatively measuring the binding force between the various segments and several types of human cells grown in tissue culture. We are using a custom built flow cell and image analysis to determine the average shear force needed to detach the cells. This is accomplished by allowing the cells to attach to the bottoms of a 96-well plate, which will be moved automatically from well to well under computer control.

由于已知HIV的达特蛋白会在感染后进入细胞， 体外和刺激艾滋病毒产品的生产，几乎有 在细胞膜上肯定有一个受体。 其中的片段 86个氨基酸的蛋白质正在合成，以确定哪个片段 负责与受体结合。 这一现象将在 通过定量测量在所述膜之间的结合力来评价所述膜。 组织中生长的各种片段和几种类型的人体细胞 文化 我们使用定制的流动池和图像分析， 测定分离细胞所需的平均剪切力。 这是 通过允许细胞附着在96孔板的底部来实现 在计算机的控制下， 控制