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KINETICS OF ERYTHROCYTE SICKLING IN PHYSIOLOGICAL CONDITIONS

KINETICS OF ERYTHROCYTE SICKLING IN PHYSIOLOGICAL CONDITIONS
生理条件下红细胞镰变的动力学
批准号：
3896217
负责人：
A EIDSATH
金额：
$0.0万
依托单位：
NATIONAL CENTER FOR RESEARCH RESOURCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3896217
关键词：
biomedical equipment development cell sorting computer data analysis hemoglobin image processing microscopy oxygen sickle cell crisis video recording system

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英文摘要
We are using a flow cell in which the partial pressure of oxygen can be varied rapidly in order to study sickling under conditions that more resemble physiological conditions than have been used in the past. Since there is a broad distribution in the cellular hemoglobin concentration, many cells need to be analyzed simultaneously. The change in shape of the cells in the flow cell is observed under a microscope. A video camera transmits the picture to a computer, where the fraction of cells that have sickled can be automatically determined using image analysis algorithms.
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