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BINDING FORCES IN RECEPTOR MEDIATED CELL ADHESION

BINDING FORCES IN RECEPTOR MEDIATED CELL ADHESION
受体介导的细胞粘附中的结合力
批准号：
3767458
负责人：
A EIDSATH
金额：
$0.0万
依托单位：
NATIONAL CENTER FOR RESEARCH RESOURCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3767458
关键词：
biophysics cell adhesion cell membrane cell sorting human immunodeficiency virus human tissue receptor binding synthetic protein tissue /cell culture virus protein virus receptors

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英文摘要
Since it is known that the tat protein of the HIV will enter the cell in vitro and stimulate the production of HIV products, there is almost certainly a receptor site for it on the cell membrane. Segments of this 86 amino acid protein are being synthesized to determine which segment is responsible for binding to the receptor. This phenomenon will be evaluated by quantitatively measuring the binding force between the various segments and several types of human cells grown in tissue culture. We hope to use a flow cell and image analysis to determine the average shear force needed to detach the cells. The cells will then be attached to the bottom of a 96-well plate, which will be moved automatically from well to well under computer control.
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国内基金
海外基金
GMFG/F-actin/cell adhesion 轴驱动 EHT 在造 血干细胞生成中的作用及机制研究
  • 批准号：
    TGY24H080011
  • 项目类别：
    省市级项目
  • 资助金额：
    --
  • 批准年份：
    2024
  • 负责人：
    李鸿鹄
  • 依托单位：
    浙江大学