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BINDING FORCES IN RECEPTOR MEDIATED CELL ADHESION

BINDING FORCES IN RECEPTOR MEDIATED CELL ADHESION
受体介导的细胞粘附中的结合力
批准号：
3874221
负责人：
A EIDSATH
金额：
$0.0万
依托单位：
NATIONAL CENTER FOR RESEARCH RESOURCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3874221
关键词：
cell adhesion cell sorting human immunodeficiency virus 1 human tissue imaging /visualization /scanning receptor binding virus protein

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英文摘要
Since it is known that the tat protein of the HIV will enter the cell in vitro and stimulate the production of HIV products, there is almost certainly a receptor site for it on the cell membrane. Segments of this 86 amino acid protein are being synthesized to determine which segment is responsible for the binding to the receptor. This will be evaluated by quantitatively measuring the binding force between several types of human cells grown in tissue culture and the various segments. We hope to use a flow cell and image analysis to determine the average shear force needed to detach cells. The cells will be attached to the bottom of a 96 well plate, which will be moved automatically from well to well under computer control.
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