KINETICS OF ERYTHROCYTE SICKLING IN PHYSIOLOGICAL CONDITIONS

生理条件下红细胞镰变的动力学

• 批准号: 3937337
• 负责人: A EIDSATH
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3937337
• 关键词: blood disorder diagnosis; cell morphology; computer data analysis; erythrocytes; flow cytometry; hemoglobin; human tissue; image processing; sickle cell crisis

We plan to build a flow cell in which the partial pressure of oxygen can be varied rapidly in order to study sickling under conditions that more resemble physiological conditions than have been used in the past. Since there is a broad distribution in the cellular hemoglobin concentration, a large number of cells need to be analyzed simultaneously. The change in shape of the cells in the flow cell will be observed under a microscope. A video camera will transmit the picture to a computer, where the fraction of cells that have sickled will be automatically determined using image analysis algorithms.

我们计划建造一个流动池， 氧气可以迅速变化，以研究镰状化， 更像生理条件的条件比有 在过去使用过。 由于在世界范围内分布广泛， 细胞血红蛋白浓度，大量的细胞需要 同时进行分析。 细胞形状的变化 在显微镜下观察流动池。 摄像机 会将图片传送到电脑， 镰状化的细胞将被自动确定， 图像分析算法