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KINETICS OF ERYTHROCYTE SICKLING IN PHYSIOLOGICAL CONDITIONS

KINETICS OF ERYTHROCYTE SICKLING IN PHYSIOLOGICAL CONDITIONS
生理条件下红细胞镰变的动力学
批准号：
3937337
负责人：
A EIDSATH
金额：
$0.0万
依托单位：
DIVISION OF RESEARCH SERVICES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3937337
关键词：
blood disorder diagnosis cell morphology computer data analysis erythrocytes flow cytometry hemoglobin human tissue image processing sickle cell crisis

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英文摘要
We plan to build a flow cell in which the partial pressure of oxygen can be varied rapidly in order to study sickling under conditions that more resemble physiological conditions than have been used in the past. Since there is a broad distribution in the cellular hemoglobin concentration, a large number of cells need to be analyzed simultaneously. The change in shape of the cells in the flow cell will be observed under a microscope. A video camera will transmit the picture to a computer, where the fraction of cells that have sickled will be automatically determined using image analysis algorithms.
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