TIME RESOLVED VIBRATIONAL SPECTROSCOPY OF HALORHODOPSIN

描述：研究光晕视紫红质的光循环，氯化物泵， 具有FTIR以及FT可见光和FT拉曼光谱。客户的续订请求 以前的3年奖，现在作为修订后的提案返回到BBCA。 具体目标是解决HR的光循环问题。检查是否或 不是希夫碱基频率依赖于卤化物，也不是 来自精氨酸侧基的带也依赖于卤素。然后, 尝试识别哪些参数出现在IR差异中 频谱。最后一个目标是获得响应的红外差分光谱 使用ATR电池，在溶液中有卤化物盐和没有卤化物盐 流动的离子。一种新的更快(5微秒)的FT-IR光谱仪 刚安装好。这将提供大约三个数量级的 更好的分辨率。

DESCRIPTION: Studying the photocycle of halo rhodopsin, a chloride pump, with FTIR as well as FT visible and FT Raman. Renewal request of a previous 3 year award, now back to BBCA as an amended proposal. Specific aims are to resolve the photocycle of hR. Examine whether or not the Schiff base frequency is halide dependent and whether or not bands from the arginine side groups are also halide dependent. Then, try to identify which args are those showing up in the IR difference spectrum. One last goal is to obtain IR difference spectra in response to, with and without halide salts in solution, using an ATR cell and flowing the ions. A new faster (5 microsecond) FT-IR spectrometer has just been installed. This will provide about three orders of magnitude better resolution.