TIME RESOLVED VIBRATIONAL SPECTROSCOPY OF HALORHODOPSIN

盐视紫红质的时间分辨振动光谱

• 批准号: 2841050
• 负责人: MARK S BRAIMAN
• 金额: $ 7.65万
• 依托单位: SYRACUSE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2841050
• 关键词: Raman spectrometry; chloride channels; gene mutation; infrared spectrometry; interferometry; protein structure function; rhodopsin; site directed mutagenesis; time resolved data

DESCRIPTION: Studying the photocycle of halo rhodopsin, a chloride pump, with FTIR as well as FT visible and FT Raman. Renewal request of a previous 3 year award, now back to BBCA as an amended proposal. Specific aims are to resolve the photocycle of hR. Examine whether or not the Schiff base frequency is halide dependent and whether or not bands from the arginine side groups are also halide dependent. Then, try to identify which args are those showing up in the IR difference spectrum. One last goal is to obtain IR difference spectra in response to, with and without halide salts in solution, using an ATR cell and flowing the ions. A new faster (5 microsecond) FT-IR spectrometer has just been installed. This will provide about three orders of magnitude better resolution.

描述：研究晕视紫红质的光循环，氯化物泵，

项目成果

Differences between the photocycles of halorhodopsin and the acid purple form of bacteriorhodopsin analyzed with millisecond time-resolved FTIR spectroscopy.

使用毫秒时间分辨 FTIR 光谱分析盐菌视紫红质和酸性紫色形式的细菌视紫红质的光循环之间的差异。

• DOI: 10.1016/0301-4622(95)00023-q
• 发表时间: 1995
• 期刊: Biophysical chemistry
• 影响因子: 3.8
• 作者: Mitrovich,QM;Victor,KG;Braiman,MS
• 通讯作者: Braiman,MS

Nano- and microsecond time-resolved FTIR spectroscopy of the halorhodopsin photocycle.

盐视紫红质光循环的纳秒和微秒时间分辨 FTIR 光谱。

• DOI: 10.1111/j.1751-1097.1997.tb03220.x
• 发表时间: 1997
• 期刊: Photochemistry and photobiology
• 影响因子: 3.3
• 作者: Dioumaev,AK;Braiman,MS
• 通讯作者: Braiman,MS