FOURIER TRANSFORM INFRARED/RAMAN SPECTROMETER

傅里叶变换红外/拉曼光谱仪

• 批准号: 2284216
• 负责人: MARK S BRAIMAN
• 金额: $ 15.15万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-27 至 1995-07-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2284216
• 关键词: biomedical equipment purchase; digital imaging; infrared spectrometry; interferometry; lasers; membrane structure; structural biology; time resolved data

A state=of-the-art- Fourier-transform (FT) infrared/Raman spectrometer will be used o perform vibrational spectroscopic studies of biomolecules. The instrument selected, a Digilab FTS60A, is capable of step-scanning interferograms obtained either from broadband IR light that is attenuated at selected wavelengths by a thin biological sample, or from near-IR light from an intense 1064-nm laser that is scattered and shifted to a variety of longer wavelengths via the Raman effect. The step-scanning capability of the instrument, along with its fast (400 KHz) digitizer with 19-bit dynamic range, will make it possible to obtain broadband time-resolved IR absorption and Raman scattering measurements with time resolution as fast as 5-mus. Furthermore, using a high- repetition-rate Q-switched Nd+-YAG laser should permit time resolution for Raman experiments down into the 10 nsec range. The beamsplitter and detector combination to be obtained along with spectrometer will allow complete spectral coverage from 10,000-100 cm in the infrared, and of measurements of Stokes-shifted Raman bands from 4,000-75cm. A microscope accessory for the FTS60A will allow sensitive IR absorbance difference measurements to be made on tiny quantities of sample (1 ng). The NIH-supported core group is planning to use the spectrometer principally to examine the structure and dynamics of biomembrane components, including simple lipid bilayer systems, supported planar bilayers with small peptides bound to them, and moderately large membrane proteins reconstituted into lipid vesicles. The proposed core users have all independently published NIH-supported papers demonstrating a capability and interest in using FT-IR and/or Raman spectroscopy to answer structural questions about membrane components involved in a variety of biomedically-important processes such as visual transduction, transmembrane chloride transport, and influenza virus insertion into cell membranes.

最先进的傅里叶变换（FT）红外/拉曼光谱仪

项目成果

Vibrational spectra of individual millimeter-size membrane patches using miniature infrared waveguides.

使用微型红外波导的单个毫米尺寸膜片的振动光谱。

• DOI: 10.1016/s0006-3495(97)78254-7
• 发表时间: 1997
• 期刊: Biophysical journal.
• 作者: Plunkett,SE;Jonas,RE;Braiman,MS
• 通讯作者: Braiman,MS