TIME RESOLVED VIBRATIONAL SPECTROSCOPY OF HALORHODOPSIN

盐视紫红质的时间分辨振动光谱

• 批准号: 2392162
• 负责人: MARK S BRAIMAN
• 金额: $ 10.74万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2392162
• 关键词: Raman spectrometry; chloride channels; gene mutation; infrared spectrometry; interferometry; protein structure function; rhodopsin; site directed mutagenesis; time resolved data

DESCRIPTION: Studying the photocycle of halo rhodopsin, a chloride pump, with FTIR as well as FT visible and FT Raman. Renewal request of a previous 3 year award, now back to BBCA as an amended proposal. Specific aims are to resolve the photocycle of hR. Examine whether or not the Schiff base frequency is halide dependent and whether or not bands from the arginine side groups are also halide dependent. Then, try to identify which args are those showing up in the IR difference spectrum. One last goal is to obtain IR difference spectra in response to, with and without halide salts in solution, using an ATR cell and flowing the ions. A new faster (5 microsecond) FT-IR spectrometer has just been installed. This will provide about three orders of magnitude better resolution.

描述：研究卤视紫红质的光循环，一种氯离子泵， 用FTIR以及FT可见光和FT拉曼。 申请延长 前3年奖，现在回到BBCA作为修改后的建议。 具体的目标是解决光循环的HR。检查是否或 不是席夫碱频率依赖于卤化物， 来自精氨酸侧基的谱带也是卤化物依赖性的。 然后， 尝试识别哪些参数出现在IR差异中 江西篇章 最后一个目标是获得响应中的IR差谱 在溶液中有和没有卤化物盐的情况下，使用ATR池， 使离子流动。 一种新的更快（5微秒）的FT-IR光谱仪， 刚刚安装。 这将提供大约三个数量级的 更好的分辨率。