盐视紫红质的时间分辨振动光谱-猫眼课题宝

盐视紫红质的时间分辨振动光谱

批准号：

2392162

负责人：

MARK S BRAIMAN

金额：

$10.74万

依托单位：

UNIVERSITY OF VIRGINIA

依托单位国家：

美国

项目类别：

财政年份：

1992

资助国家：

美国

项目状态：

已结题

起止时间：

1992-09-30 至 1999-03-31

来源：

https://reporter.nih.gov/project-details/2392162

关键词：

Raman spectrometry chloride channels gene mutation infrared spectrometry interferometry protein structure function rhodopsin site directed mutagenesis time resolved data

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项目成果

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中文摘要

翻译

描述：研究晕视紫红质的光循环，氯化物泵，

英文摘要

DESCRIPTION: Studying the photocycle of halo rhodopsin, a chloride pump, with FTIR as well as FT visible and FT Raman. Renewal request of a previous 3 year award, now back to BBCA as an amended proposal. Specific aims are to resolve the photocycle of hR. Examine whether or not the Schiff base frequency is halide dependent and whether or not bands from the arginine side groups are also halide dependent. Then, try to identify which args are those showing up in the IR difference spectrum. One last goal is to obtain IR difference spectra in response to, with and without halide salts in solution, using an ATR cell and flowing the ions. A new faster (5 microsecond) FT-IR spectrometer has just been installed. This will provide about three orders of magnitude better resolution.

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