BIOPHYSICS OF INFLUENZA HEMAGGLUTININ MEDIATED FUSION

描述 该研究提出希望了解发生在 流感病毒HA蛋白介导膜时的脂双层 核聚变 荧光共振能量转移（FRET），偏振 各向异性和膜片钳测量将用于监测 在融合过程中病毒和靶细胞膜。 更改将 使用一组已经合成的荧光探针进行监测， 由外国合作者开发。 具体目标是：1. 在病毒结合和膜融合过程中神经节苷脂聚集， 将研究通过降低pH值引发的。 2. 病毒之间的距离 并评价融合前状态的脂质体膜。 3. 的 神经节苷脂结合的HA和未结合的HA在引起融合中的作用将是 测定 4. 内外脂混合动力学 将比较病毒-脂质体融合期间的单层。 5. 的影响 脂质体中脂质组成对脂质混合动力学的影响将 被研究。 6. 表达HA的细胞与表达HA的细胞之间的孔生长动力学 将测量大的单层囊泡。

DESCRIPTION The research proposed desires to understand the events occurring within the lipid bilayer when the HA protein of influenza virus mediates membrane fusion. Fluorescence resonance energy transfer (FRET), polarization anisotropy and patch clamp measurements will be used to monitor changes in both viral and target membranes during the fusion process. Changes will be monitored using a set of fluorescent probes that have been synthesized and developed by the foreign collaborators. The specific aims are: 1. Ganglioside clustering during virus binding and membrane fusion that is triggered by lowering pH will be studied. 2. The distances between virus and liposome membranes in the prefusion state will be evaluated. 3. The role of ganglioside-bound HA and unbound HAs in causing fusion will be determined. 4. The kinetics of mixing of the inner and outer lipid monolayers during virus-liposome fusion will be compared. 5. The influence of the lipid composition in liposomes on the kinetics of lipid mixing will be studied. 6. The kinetics of pore growth between HA-expressing cells and large unilamellar vesicles will be measured.