TARGETED MUTAGENESIS OF DNA VIA TRIPLE HELIX FORMATION

描述：这项工作的总体目标是开发和测试新的 用遗传学的方法来检验三螺旋形成的假说 寡核苷酸可用于递送位点选择性DNA损伤， 哺乳动物细胞中的突变。 这项工作的具体目标是 开发新的模型来研究三螺旋介导的突变（基于 航天飞机载体技术），以研究物理参数 其在体外影响这些穿梭载体的靶向诱变， 研究影响TFO介导的DNA损伤和修复途径 突变，最后将工作扩展到研究TFO介导的突变 在小鼠的染色体基因中，而不是穿梭载体。

DESCRIPTION: The overal goal of the work is to develop and test novel genetic methods to test the hypothesis that triple helix forming oligonucleotides can be used to deliver site-selective DNA damage and mutation in mammalian cells. The specific aims of the work are to develop new models to study triple helix- mediated mutagenesis (based upon shuttle vector technology), to investigate the physical parameters which affect targeted mutagenesis upon these shuttle vectors in vitro, to examine DNA damage and repair pathways which influence TFO mediated mutation, and finally to extend the work to study TFO mediated mutation in chromosomal genes of the mouse, rather than shuttle vectors.