LEISHMANIA ANTIGENS--BIOCHEMICAL AND IMMUNOLOGIC STUDIES

利什曼原虫抗原——生物化学和免疫学研究

• 批准号: 2607754
• 负责人: Diane McMahon Pratt
• 金额: $ 37.37万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2607754
• 关键词: Leishmania; active immunization; cell population study; clone cells; cytokine; epitope mapping; genetic manipulation; genetic regulation; glycoproteins; immunocytochemistry; immunomodulators; intracellular parasitism; laboratory mouse; laboratory rabbit; leishmaniasis; life cycle; membrane proteins; microorganism genetics; microorganism immunology; molecular genetics; protozoal antigen; protozoal vaccine; vaccine development; vaccinia virus

The purpose of this proposal is to continue and extend the investigations initiated in the previous period of support, focusing on the GP46/M-2 gene family of Leishmania. The objectives should determine the immunological mechanisms involved in protective immunity elicited by the GP46/M-2 membrane surface glycoprotein. Based on the fact that immunization with GP46/M-2 appears to effect an early phase(s) of infection (resulting in either a delay in onset or complete protection), these studies will focus on the early immunological events occurring in response to infection in immunized/non-immunized mice. Further, this proposal seeks to employ recent genetic as well as biochemical approaches to determine the biological role of the GP46/M-2 family in vivo. Specifically the experimental approaches proposed are: 1. To determine the effector cells, mediators and mechanisms responsible for protection against infection induced by immunization with GP46/M-2 and the adjuvant, Corynebacterium parvum. Experimental approaches will employ cellular reconstitution (using cloned T cell lines) and immunohistochemical analyses at the site of infection. Epitope mapping will determine protective epitopes for potential use in a peptide subunit vaccine. 2. To determine the mechanism(s) (cell subpopulations, cytokines) involved in protection due to immunization using GP46/M-2-vaccinia recombinant viruses. The ability of recombinant GP46-vaccinia virus to induce protection against infection with L. major and L. donovani will also be evaluated. In addition, the potential of recombinant non-pathogenic vaccinia and canary pox viruses in the induction of a protective immune response against infection with Leishmania species will be investigated. 3. To investigate the structure (biochemical), subcellular distribution, gene regulation (at the mRNA level) and biological function of various members of the GP46/M-2 gene family. Functional studies, employing a molecular genetic approach, will focus on development of the parasite within the phlebotomine sand fly vector.

这项建议的目的是继续和扩大调查

项目成果

Leishmaniasis and its etiologic agents in the New World: an overview.

新大陆利什曼病及其病原体：概述。

• 发表时间: 1991
• 期刊: Progress in clinical parasitology
• 作者: GrimaldiJr,G;McMahan-Pratt,D
• 通讯作者: McMahan-Pratt,D

Production of a specific monoclonal antibody for the identification of Leishmania (Leishmania) venezuelensis.

生产用于鉴定委内瑞拉利什曼原虫（Leishmania）的特异性单克隆抗体。

• DOI: 10.4269/ajtmh.1990.42.453
• 发表时间: 1990
• 期刊: The American journal of tropical medicine and hygiene
• 作者: Hanham,CA;Shaw,JJ;Lainson,R;McMahon-Pratt,D
• 通讯作者: McMahon-Pratt,D

Andean leishmaniasis in Ecuador caused by infection with Leishmania mexicana and L. major-like parasites.

厄瓜多尔的安第斯利什曼病是由墨西哥利什曼原虫和类利什曼原虫感染引起的。

• DOI: 10.4269/ajtmh.1991.44.205
• 发表时间: 1991
• 期刊: The American journal of tropical medicine and hygiene
• 作者: Hashiguchi,Y;Gomez,EA;deCoronel,VV;Mimori,T;Kawabata,M;Furuya,M;Nonaka,S;Takaoka,H;Alexander,JB;Quizhpe,AM
• 通讯作者: Quizhpe,AM

Evolution of the genus Leishmania as revealed by comparisons of nuclear DNA restriction fragment patterns.

通过比较核 DNA 限制性片段模式揭示利什曼原虫属的进化。

• DOI: 10.1073/pnas.84.2.484
• 发表时间: 1987
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Beverley,SM;Ismach,RB;Pratt,DM
• 通讯作者: Pratt,DM

Presentation via the class I pathway by Leishmania amazonensis-infected macrophages of an endogenous leishmanial antigen to CD8+ T cells.

亚马逊利什曼原虫感染的巨噬细胞通过 I 类途径将内源性利什曼原虫抗原呈递给 CD8 T 细胞。

• 发表时间: 1997
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Kima,PE;Ruddle,NH;McMahon-Pratt,D
• 通讯作者: McMahon-Pratt,D