52KD RO/SSA AUTOANTIGEN

52KD RO/SSA 自身抗原

• 批准号: 2769595
• 负责人: Mark Frank
• 金额: $ 15.41万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2769595
• 关键词: DNA binding protein; autoantibody; autoantigens; clinical research; genetic polymorphism; genetic promoter element; human subject; molecular pathology; nucleic acid sequence; systemic lupus erythematosus

DESCRIPTION (Adapted from Investigator's abstract): Autoantibodies to a 52kd Ro/SSA protein occur in some patients with SLE, and are inversely correlated with renal disease in Black American SLE patients. A polymorphism of the 52kd Ro gene correlates with this finding, leading to the hypotheses in this project that, first, this polymorphism may be a genetic marker for an allelic variant of 52kd Ro which preferentially occurs in lupus patients, or secondly, that this polymorphism is a marker for a mutation which disregulates the expression of this gene. Sequence analysis and functional promoter studies will be used in Aim 1 to address these hypotheses. In Aim 2, the DNA binding properties of the 52kd Ro protein will be investigated and the possible role of this protein influencing gene expression will be studied. Aim 3 will quantitate antibodies to 52kd Ro in subsets of lupus patients and Aim 4 will study the pathogenicity of these autoantibodies focusing on lesions of cutaneous lupus and binding of these autoantibodies to kerotinocytes after UV irradiation, a trigger for the photosensitive reactions of cutaneous lupus patients.

描述（改编自研究者摘要）：抗 52 kd Ro/SSA蛋白存在于部分SLE患者中，与SLE患者的SLE发病率呈负相关。 与美国黑人SLE患者的肾脏疾病相关。 一 52 kd Ro基因的多态性与这一发现相关，导致 在这个项目中的假设，首先，这种多态性可能是一个 52 kd Ro等位基因变异体的遗传标记， 在狼疮患者中，或者第二，这种多态性是一种标志物， 突变，使该基因的表达失调。 序列分析 和功能启动子研究将用于目标1，以解决这些问题 假设 目的2研究了52 kd Ro蛋白与DNA的结合特性， 将进行研究，这种蛋白质影响基因的可能作用 表达将被研究。 目的3将定量抗体52 kd Ro， 亚群的狼疮患者和目标4将研究这些致病性 自身抗体集中在皮肤狼疮病变和结合这些 紫外线照射后角质形成细胞的自身抗体， 皮肤狼疮患者的光敏反应。

项目成果

Characterization of DNA binding properties and sequence specificity of the human 52 kDa Ro/SS-A (Ro52) zinc finger protein.

人类 52 kDa Ro/SS-A (Ro52) 锌指蛋白的 DNA 结合特性和序列特异性的表征。

• DOI: 10.1006/bbrc.1999.0835
• 发表时间: 1999
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Frank,MB