CORE--ANALYTICAL CHEMISTRY

核心--分析化学

• 批准号: 6269053
• 负责人: Daniel C Liebler
• 金额: $ 14.87万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-14 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6269053
• 关键词: analytical chemistry; biomedical facility; high performance liquid chromatography; mass spectrometry; protein sequence

The Analytical Core Shared Service provides state of the art instrumentation for mass spectrometry-based chemical and biochemical analyses and personnel to assist investigators in the design and application of analytical methods and the interpretation of data. The Analytical Core is supported both by the Arizona Cancer Center (ACC) and the Southwest Environmental Health Sciences Center (SWEHSC) core grants. The objectives of the Analytical Core are to aid ACC and SWEHSC investigators: . In the identification of unknown compounds, . In the development of mass spectrometry based analytical methods, and . In the design of experiments and interpretation of analytical data. The Analytical Core is directed by Daniel C. Liebler, Ph.D., Associate Professor of Pharmacology and Toxicology and staffed by Thomas D. McClure, Ph.D., facility manager and one research specialist each supported by the ACC and SWEHSC core grants. Instrumentation in the Analytical Core includes a Finnigan TSQ7000 triple quadrupole instrument with electrospray and atmospheric pressure chemical ionization for HPLC-MS and HPLC-MS-MS work. This instrument is capable of protein and peptide sequence analysis as well as the analysis of a wide variety of biomolecules and macromolecules. The Analytical Core also houses a Finnigan MD-800 bench- top GC-MS system and a Hewlett Packard 1050 diode array HPLC system for method development. All analytical work is done by appointment. There is no charge assessed to ACC and SWEHC investigators for method development, whereas a flat per-sample fee is charge to recover costs of instrument service contracts.

分析核心共享服务提供最先进的 基于质谱的化学和生物化学仪器 分析和人员，以协助研究人员在设计和 分析方法的应用和数据的解释。的 Analytical Core由亚利桑那州癌症中心（ACC）和 西南环境健康科学中心（SWEHSC）的核心赠款。 分析核心的目标是帮助ACC和SWEHSC 研究者： .在鉴定未知化合物时， .在基于质谱的分析方法的开发中， .在实验设计和分析数据的解释。 分析核心由丹尼尔C。利布勒博士，副 药理学和毒理学教授，由托马斯D.麦克卢尔， 哲学博士、设施经理和一名研究专家， 行政协调会和SWEHSC核心赠款。分析核心中的仪器 包括Finnigan TSQ 7000三重四极杆仪器， HPLC-MS和HPLC-MS-MS的大气压化学电离 工作该仪器能够进行蛋白质和肽序列分析 以及各种生物分子的分析， 大分子分析核心还设有一个芬尼根MD-800工作台- 顶部GC-MS系统和Hewlett Packard 1050二极管阵列HPLC系统， 方法开发所有的分析工作都是预约完成的。有 ACC和SWEHC研究者无需承担方法开发费用， 而每个样本收取固定费用，以收回仪器成本 服务合同。