MOLECULAR EFFECTS OF ELF FIELDS WITH HARMONIC CONTENTS

本提案是根据以下要求编写的： 申请RFA ES-096-07，标题为“生物效应发生频率 电磁场特别是，我们回应了对“ 暴露条件的潜在生物效应，如谐波” 以及研究这种电磁场的遗传效应。我们建议 60-Hz诱发即刻早期基因转录研究 电磁场（EMF）具有更高的谐波含量。的 现有的基于电磁铁的磁场暴露系统将被供电 通过方波电输入，而不是正弦波电输入。 我们将检验暴露于高频电磁场的假设- 组分改变立即早期基因的表达，因此， 存在电磁场引起的不良健康影响的风险，包括 癌 除了60 °外还包括谐波的宽频电磁场 Hz在工作和其他环境中是常见的。 目前的实验 数据库不包括频谱作为变量。 职业 或家庭暴露于这些领域，如果生物活性， 对人类健康构成长期威胁。 我们会利用基因 转录作为EMF诱导反应的模型。 具体目标 的建议是确定差异表达的已知和新的 人HL 60或MCF 7细胞在2 mT、3或 在暴露后0和3 h暴露24 h。 我们已经开发 并成功测试了一种基于聚合酶链反应的技术， 快速互补DNA（cDNA）筛选和分离 从一个随机选择的基因中差异表达的基因， 细胞特异性cDNA文库，其量足够大以用于测序。

This proposal has been prepared in response to the request for application RFA ES-096-07 entitled "Biological Effects Poser Frequency EMF." In particular, we respond to the need for research on "the potential biological effects for exposure conditions such as harmonics" and for research on genetic effects of such EMFs. We propose to investigate immediate early gene transcription induced by 60-Hz electromagnetic fields (EMF) with a higher harmonic content. The existing solenoid-based magnetic field exposure system will be powered by a square-wave electric input, instead of a sine-wave electric input. We will test the hypothesis that exposure to EMFs with higher frequency- components alter expression of immediate early genes and, therefore, that there is a risk for EMF-induced adverse health effects, including cancer. Broad frequency EMFs that include harmonics in addition to 60 Hz are common n work and other environments. The current experimental database does not include frequency spectra as a variable. Occupational or domestic exposure to such fields, if biologically active, may represent a long-term health risk to humans. We will use gene transcription as a model of an EMF-induced response. The specific aim of the proposal is to identify differentially expressed known and novel immediate early genes in human HL60 or MCF7 cells following 2 mT, 3 or 24 h exposure at post-exposure times of 0 and 3 h. We have developed and successfully tested a polymerase chain reaction-based technique for the rapid complementary DNA (cDNA) screening and isolation of differentially expressed genes among randomly-selected genes from a cell-specific cDNA library in large enough quantities for sequencing.