MECHANISM OF MICROWAVE CARCINOGENESIS IN VITRO

根据我们最新的体外实验证据表明，它具有致癌作用 2.45 GHz微波，我们建议评估微波的影响，在 无兆瓦供暖(兆瓦期间温升低于0.3摄氏度 细胞暴露)，对体外致癌转化的影响 共应激源[X射线，肿瘤促进剂12-0-十四酰-佛波醇-13-乙酸酯 (TPA)]。我们的实验目的是：(A)阐明相互作用 在细胞水平上导致致癌效应的机制，(B) 根据比表面积建立微波剂量-效应关系 致癌效应吸收率(SAR)。 我们将用小鼠胚胎细胞来源的C3H/10T1/2细胞系 哪个转换通过对堆积焦点在 融合的单层。实验将在37摄氏度的条件下进行 积极发展的文化。微波辐射对体外培养的C3H/10T1/2细胞的影响 将通过比较诱导的转化子的数量来衡量 持续24小时的自由场微波照射(SAR=0.1~10W/kg) 有或没有后续处理后培养在0.1g/ml的TPA中用于UP 到8周。平行实验将包括使用分级剂量的X 射线作为与微波联合转化的第二引发剂 和肿瘤促进剂来检验假设，长期接触 微波可以增强和诱导其他致癌物的作用。这个 理解非热效应的细胞机制的重要性 兆波场与生物系统的相互作用是很重要的，因为 这样的场在环境中无处不在。

On the basis of our recent in vitro evidence for the carcinogenic action of 2.45 GHz microwaves (MW), we propose to assess the effect of MW, in the absence of MW heating (less than 0.3 degrees C temperature rise during MW cell exposure), on in vitro oncogenic transformation with or without other co-stressors [X rays, tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate (TPA)]. Our experiments aim at: (a) the elucidation of interaction mechanisms at the cellular level leading to oncogenic effects, (b) the establishment of the MW dose-response relationship in terms of the specific absorption rate (SAR) for oncogenic effects. We will use the C3H/10T1/2 cell line derived from mouse embryo cells in which transformation is quantitated by scoring piled-up foci on top of a confluent monolayer. Experiments will be performed at 37 degrees C with actively growing cultures. The effect of MW on C3H/10T1/2 cells in culture will be measured by comparing the number of transformants induced by free-field microwave irradiation lasting for 24 h (SAR = 0.1 to 10 W/kg) with or without subsequent post-treatment culture in TPA at 0.1 g/ml for up to 8 weeks. Parallel experiments will include the use of graded doses of X rays as a second initiator of transformation in conjunction with microwaves and tumor promoter to test hypothesis that the prolonged exposure to microwaves can enhance and elicit effect of other carcinogens. The importance of understanding of the cellular mechanisms whereby non-thermal MW fields interact with biological systems is important due to the pervasive presence of such fields in the environment.