MOLECULAR EFFECTS OF ELF FIELDS WITH HARMONIC CONTENTS

本提案是应以下要求编写的： 应用RFA ES-096-07题为“生物效应频率” 尤其是，我们回应了研究的需要“ 谐波等暴露条件的潜在生物效应“ 并对这种电磁场的遗传效应进行研究。我们建议 研究60赫兹辐射诱导的即刻早期基因转录 具有较高谐波含量的电磁场。这个 现有的基于螺线管的磁场曝光系统将被供电 通过方波输入，而不是正弦波电输入。 我们将测试这一假设，即暴露在频率更高的电磁场中- 成分改变即刻早期基因的表达，因此， 存在电磁场引起的不良健康影响的风险，包括 癌症。宽频电磁场，除60次谐波外，还包括谐波 赫兹在工作和其他环境中很常见。目前的实验 数据库不包括作为变量的频谱。职业 或国内暴露在这样的领域，如果生物活跃，可能 对人类构成长期的健康风险。我们将使用基因 转录作为电磁场诱导反应的模型。具体目标 该提案的目的是识别不同表达方式的已知和新奇 人HL60或MCF7细胞中即刻早期基因的表达 在暴露后0和3h的24小时暴露。我们已经开发出 并成功地测试了一种基于聚合酶链式反应的技术 沙门氏菌快速互补DNA(CDNAs)的筛选和分离 在随机选择的基因中差异表达的基因 有足够数量的细胞特异的cDNA库进行测序。

This proposal has been prepared in response to the request for application RFA ES-096-07 entitled "Biological Effects Poser Frequency EMF." In particular, we respond to the need for research on "the potential biological effects for exposure conditions such as harmonics" and for research on genetic effects of such EMFs. We propose to investigate immediate early gene transcription induced by 60-Hz electromagnetic fields (EMF) with a higher harmonic content. The existing solenoid-based magnetic field exposure system will be powered by a square-wave electric input, instead of a sine-wave electric input. We will test the hypothesis that exposure to EMFs with higher frequency- components alter expression of immediate early genes and, therefore, that there is a risk for EMF-induced adverse health effects, including cancer. Broad frequency EMFs that include harmonics in addition to 60 Hz are common n work and other environments. The current experimental database does not include frequency spectra as a variable. Occupational or domestic exposure to such fields, if biologically active, may represent a long-term health risk to humans. We will use gene transcription as a model of an EMF-induced response. The specific aim of the proposal is to identify differentially expressed known and novel immediate early genes in human HL60 or MCF7 cells following 2 mT, 3 or 24 h exposure at post-exposure times of 0 and 3 h. We have developed and successfully tested a polymerase chain reaction-based technique for the rapid complementary DNA (cDNA) screening and isolation of differentially expressed genes among randomly-selected genes from a cell-specific cDNA library in large enough quantities for sequencing.