MECHANISM OF MICROWAVE CARCINOGENESIS IN VITRO

根据我们最近的体外证据， 2.45 GHz微波（MW），我们建议评估MW的影响，在 没有MW加热（MW期间温度上升小于0.3 ℃） 细胞暴露），在有或没有其他 共应激物[X射线，肿瘤促进剂12-0-十四酰基-佛波醇-13-乙酸酯 （TPA）]。 我们的实验旨在：（a）阐明相互作用 在细胞水平导致致癌作用的机制，（B） 在特定的剂量反应关系方面， 致癌效应的吸收率（SAR）。 我们将使用来自小鼠胚胎细胞的C3 H/10 T1/2细胞系， 该变换是通过对堆积在 融合单层。 实验将在37摄氏度下进行， 积极培育文化。 MW对培养的C3 H/10 T1/2细胞的影响 将通过比较转化体的数目来测量， 持续24 h的自由场微波辐照（SAR = 0.1 - 10 W/kg） 在TPA中以0.1 μ g/ml进行或不进行后续后处理培养， 到8周。 平行实验将包括使用分级剂量的X射线 射线作为与微波结合的第二种转变引发剂 和肿瘤促进剂来检验假设， 微波可以增强和引发其他致癌物的影响。 的 理解非热传导的细胞机制的重要性 MW场与生物系统的相互作用是重要的， 这种场在环境中的普遍存在。

On the basis of our recent in vitro evidence for the carcinogenic action of 2.45 GHz microwaves (MW), we propose to assess the effect of MW, in the absence of MW heating (less than 0.3 degrees C temperature rise during MW cell exposure), on in vitro oncogenic transformation with or without other co-stressors [X rays, tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate (TPA)]. Our experiments aim at: (a) the elucidation of interaction mechanisms at the cellular level leading to oncogenic effects, (b) the establishment of the MW dose-response relationship in terms of the specific absorption rate (SAR) for oncogenic effects. We will use the C3H/10T1/2 cell line derived from mouse embryo cells in which transformation is quantitated by scoring piled-up foci on top of a confluent monolayer. Experiments will be performed at 37 degrees C with actively growing cultures. The effect of MW on C3H/10T1/2 cells in culture will be measured by comparing the number of transformants induced by free-field microwave irradiation lasting for 24 h (SAR = 0.1 to 10 W/kg) with or without subsequent post-treatment culture in TPA at 0.1 g/ml for up to 8 weeks. Parallel experiments will include the use of graded doses of X rays as a second initiator of transformation in conjunction with microwaves and tumor promoter to test hypothesis that the prolonged exposure to microwaves can enhance and elicit effect of other carcinogens. The importance of understanding of the cellular mechanisms whereby non-thermal MW fields interact with biological systems is important due to the pervasive presence of such fields in the environment.