GENE EXPRESSION FOLLOWING 60 HZ EMF EXPOSURE

描述：这项提案是为了响应国家 国家电磁场健康效应研究计划 美国国立卫生研究院环境健康科学研究所，题为“细胞 低频电磁场的影响，“要求 建议书(RFA)ES-94-001。特别是，调查人员对 需要一项研究工作，解决权力的影响- 60 Hz频率电磁场对人体基因表达的影响 细胞。 他们将测试极低频(ELF)电- 磁场(EMF)对细胞的作用与遗传成分有关 得了癌症。因此，这项提议有两个目标：1)他们将 提供转录反应的详细特征 培养的人细胞(HL-60和MCF-7)。在为此目的而提出的研究中， 他们将研究c-myc、c-fos、 野生型P53、野生型P53激活片段1和鸟氨酸 脱羧酶(ODC)基因在几种暴露和暴露后的模式 使用Northern印迹和逆转录酶的暴露时间- 聚合酶链式反应(RT-PCR)；2)他们将研究差异 相似暴露对HL-60和MCF-7细胞基因表达的影响 他们已经开发了一种新的技术来 差异表达基因的快速筛选和分离。在……里面 与差异杂交或差减杂交技术相比，差异杂交或差减杂交技术 优先检测属于丰富的基因类别的基因， 他们的技术可以检测出丰富和稀缺的基因 这项技术现在通常被用来比较相关的基因 在人类食道癌的恶性进展中。建议数 AIM 1的研究将包括细胞、ELF电磁场暴露条件和结束 以前由他人调查和发布的要点，并将 为剩余的研究建立实验室基准。他们将 仅使用已建立和经过验证的分子方法以及 现有的超低频电动势曝光系统，已经建成， 表征，并在目前资助的研究中使用。

DESCRIPTION: This proposal has been prepared in response to the National Electric and Magnetic Field Health Effects Research Program, National Institute of Environmental Health Sciences, NIH, entitled "Cellular Effects of Low Frequency Electromagnetic Fields," the request for proposal (RFA) ES-94- 001. In particular, the investigators respond to the need for a research effort, which addresses the effects of power- frequency 60 Hz electromagnetic fields on gene expression in human cells. They will test the hypothesis that extremely low frequency (ELF) electro- magnetic field (EMF) action on cells has genetic components associated with cancer. Accordingly, this proposal has two aims: 1) they will provide detailed characterization of the transcriptional response of cultured human cells (HL-60 and MCF-7). In studies proposed for this aim, they will investigate messenger RNA (mRNA) expression of c-myc, c-fos, wild-type p53, wild-type p53-activated fragment 1 (WAF-1) and ornithine decarboxylase (ODC) genes at several patterns of exposure and post- exposure times using Northern blotting and reverse transcriptase- polymerase chain reaction (RT-PCR); and 2) They will study differential gene expression in HL-60 and MCF-7 cells using similar exposure conditions as for aim 1. They have developed a novel technique for rapid screening and isolation of differentially expressed genes. In contrast to differential or subtractive hybridization technique, which preferentially detects genes belonging to the abundant class of genes, their technique can detect genes in both, abundant and scarce classes.This technique is now routinely used to compare genes involved in malignant progression in human esophageal cancer. The proposed studies in aim 1 will include cells, ELF EMF exposure conditions and end points previously investigated and published by others, and will establish their laboratory baseline for the remaining studies.They will use only the established and proven molecular methods as well as an existing ELF EMF exposure system, which has been constructed, characterized, and used in the currently funded research.