CRYSTALLOGRAPHIC STUDIES OF PROTEIN STRUCTURE & FUNCTION
CRYSTALLOGRAPHIC STUDIES OF PROTEIN STRUCTURE & FUNCTION
批准号:
6123278
负责人:
GREGORY A PETSKO
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-07-01 至 1999-06-30
中文摘要
这项研究计划的总体目标是了解
镁离子在催化反应机理中的作用
碳水化合物加工金属酶。 研究人员还希望
了解蛋白质动力学如何与金属蛋白质功能结合,
稳定 为了实现这些目标,我们使用我们拥有的新方法,
在过去的几年里,包括冷冻脑血管造影术,
和时间分辨晶体学 有三个相互依存的
在这个大项目中的子项目。 第一个继续研究
木糖异构酶。 调查人员正在确定
以推断镁离子在
催化开环反应。 然后,我们将模拟
量子力学/分子生物学相结合的全催化途径
力学,以了解桥连的独特催化性能
心脏病。 第二个项目的重点是
镁在3-异丙基苹果酸催化反应中的作用
脱氢酶。 研究人员将使用X射线和
晶体学和遗传选择来绘制
热稳定性、蛋白质柔性和金属依赖性活性
在这种必需的氨基酸合成酶中。 最后我们将
确定了双官能团的结构和催化机理
铜绿假单胞菌甘露糖-6-磷酸异构酶。 这
酶催化藻酸盐生产中的两个非连续反应:
这两种反应都需要镁离子。 这三种酶都能
低温或劳厄晶体学,
E-S络合物和反应中间体可以在
或者是单一底物/单一产物酶,其中
生产性Nfichaelis复合物可以直接在
在平衡条件下结晶。
英文摘要
The overall objective of this research program is to understand
the role of magnesium ion in the catalytic mechanisms of
carbohydrate-processing metalloenzymes. Investigators also wish to
learn how protein dynamics if coupled to metalloprotein function and
stability. To achieve these aims, we use novel methods that we have
developed over the last several years, including cryocrystallography
and time-resolved crystallography. There are three interdependent
sub-projects within this overall project. The first continues studies
of xylose isomerase. The investigators are determining the structure
of the initial E-S complex to deduce therole of the magnesium ions in
catalysis of the ring-opening reaction. We will then simulate the
entire catalytic pathway by combined quantum mechanics/molecular
mechanics, to understand the unique catalytic properties of bridged
bimetallic centers. The second project focuses on the role of
magnesium in the reaction catalyzed by 3-isopropylmalate
dehydrogenase. Investigators will usea combination of X-ray
crystallography and genetic selection to map the correlations between
thermal stability, protein flexibility, and metal-dependent activity
in this essential arnino-acid synthesizing enzyme. Finally, we will
determine the structure and catalytic mechanism of the bifunctional
mannose-6-phosphate isomerase from Pseudomonay aeruginosa. This
enzyme catalyzes two non-contiguous reactions in alginate production:
Both reactions require magnesium ion. All three enzymes are amenable
to low temperature or Laue crystallography, so that transient
E-Scomplexes and reaction intermediates can be trapped and studied at
high resolution; or are single substrate/single product enzymes, where
the productive Nfichaelis complex can be observed directly in the
crystal under equilibrium conditions.
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科研奖励(0)
会议论文
STRUCTURE BIOLOGY OF ENZYMES AND DNA-BINDING PROTEINS
-
批准号:7721252
-
项目类别:
-
资助金额:$1.41万
-
财政年份:2008
-
负责人:GREGORY A PETSKO
-
依托单位:
STRUCTURE BIOLOGY OF ENZYMES AND DNA-BINDING PROTEINS
-
批准号:7369543
-
项目类别:
-
资助金额:$0.27万
-
财政年份:2005
-
负责人:GREGORY A PETSKO
-
依托单位:
TELLURIUM AS HEAVY ATOM FOR PROTEIN STRUCTURE DETERMINATION
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批准号:6120845
-
项目类别:
-
资助金额:$1.54万
-
财政年份:1999
-
负责人:GREGORY A PETSKO
-
依托单位:
X RAY GENERATOR/AREA DETECTOR FOR STRUCTURAL BIOLOGY
-
批准号:2040270
-
项目类别:
-
资助金额:$39.99万
-
财政年份:1997
-
负责人:GREGORY A PETSKO
-
依托单位:
MECHANISMS OF ENZYMIC AND HYDRIDE TRANSFERS
-
批准号:6179634
-
项目类别:
-
资助金额:$22.49万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
CRYSTALLOGRAPHIC STUDIES OF PROTEIN STRUCTURE/FUNCTION
-
批准号:2174808
-
项目类别:
-
资助金额:$22.52万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
SITE SPECIFIC MUTAGENESIS OF ISOMERASES
-
批准号:2176565
-
项目类别:
-
资助金额:$17.41万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
SITE-SPECIFIC MUTAGENESIS OF ISOMERASES
-
批准号:3281221
-
项目类别:
-
资助金额:$17.84万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
CRYSTALLOGRAPHIC STUDIES OF PROTEIN STRUCTURE/FUNCTION
-
批准号:2734414
-
项目类别:
-
资助金额:$22.11万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
CRYSTALLOGRAPHIC STUDIES OF PROTEIN STRUCTURE/FUNCTION
-
批准号:3274231
-
项目类别:
-
资助金额:$31.0万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
Structural Basis for Bridged Bimetallic Enzyme Catalysis
-
批准号:6727680
-
项目类别:
-
资助金额:$30.17万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
MECHANISMS OF ENZYMIC AND HYDRIDE TRANSFERS
-
批准号:6759437
-
项目类别:
-
资助金额:$36.77万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
MECHANISMS OF ENZYMIC AND HYDRIDE TRANSFERS
-
批准号:6385502
-
项目类别:
-
资助金额:$23.16万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
MECHANISMS OF ENZYMIC AND HYDRIDE TRANSFERS
-
批准号:6684595
-
项目类别:
-
资助金额:$36.02万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
Mechanisms of Enzymic and Hydride Transfers
-
批准号:7821369
-
项目类别:
-
资助金额:$39.04万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
MECHANISMS OF ENZYMIC AND HYDRIDE TRANSFERS
-
批准号:6914915
-
项目类别:
-
资助金额:$37.71万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
SITE SPECIFIC MUTAGENESIS OF ISOMERASES
-
批准号:2176564
-
项目类别:
-
资助金额:$16.91万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
CRYTALLOGRAPHIC STUDIES OF PROTEIN STRUCTURE AND FUNCTIO
-
批准号:2174806
-
项目类别:
-
资助金额:$30.7万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
Mechanisms of Enzymic and Hydride Transfers
-
批准号:7461369
-
项目类别:
-
资助金额:$39.19万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
MECHANISMS OF ENZYMIC AND HYDRIDE TRANSFERS
-
批准号:2902267
-
项目类别:
-
资助金额:$22.54万
-
财政年份:1990
-
负责人:GREGORY A PETSKO
-
依托单位:
海外基金