STRUCTURE BIOLOGY OF ENZYMES AND DNA-BINDING PROTEINS

酶和 DNA 结合蛋白的结构生物学

• 批准号: 7721252
• 负责人: GREGORY A PETSKO
• 金额: $ 1.41万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721252
• 关键词: Aminopeptidase; Binding; Binding Proteins; Biology; C-terminal; Carboxy-Lyases; Catalysis; Computer Retrieval of Information on Scientific Projects Database; Data; Enzymes; Folch-Pi apoprotein; Funding; Grant; Hydrogen; Institution; Location; Malignant Neoplasms; Motion; Myelin Proteolipid Protein; Numbers; Peptides; Proteins; Research; Research Personnel; Resources; Role; Source; Staging; Structure; Tyrosine; United States National Institutes of Health; base; chymotrypsin; protein structure; thiaminase II; ultra high resolution

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Data were collected for a variety of proteins at different stages of structure determination and exploitation. For tyrosine decarboxylase, heavy-atom derivative data were collected. This protein is a PLP-containing enzyme being studied as part of an ongoing project about protein stability and the structural basis of PLP catalysis. For chymotrypsin and aminopeptidase, ultra-high resolution data were collected as part of an ongoing project about the location of hydrogens in protein structures and the role of anisotropic motion in catalysis. For thiaminase, derivative data were collected in order to try to solve the structure of this cancer-related protein. Data were also collected on the C-terminal domain of the bacterial repressor DtxR with some putative bound peptides. A number of other crystals of projects getting underway were screened for diffraction.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 收集了各种蛋白质在结构确定和开发的不同阶段的数据。对于酪氨酸脱羧酶，收集了重原子导数数据。这种蛋白质是一种含有PLP的酶，作为一个正在进行的关于蛋白质稳定性和PLP催化的结构基础的项目的一部分进行研究。对于糜蛋白酶和氨基肽酶，作为正在进行的关于氢在蛋白质结构中的位置和各向异性运动在催化中的作用的项目的一部分，收集了超高分辨率数据。对于硫胺酶，我们收集了衍生数据，试图解开这种癌症相关蛋白的结构。还收集了细菌抑制物DtxR的C-末端结构域与一些可能的结合肽的数据。对正在进行的其他一些项目的晶体进行了衍射筛选。