X RAY GENERATOR/AREA DETECTOR FOR STRUCTURAL BIOLOGY

用于结构生物学的 X 射线发生器/区域探测器

• 批准号: 2040270
• 负责人: GREGORY A PETSKO
• 金额: $ 39.99万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2040270
• 关键词: X ray crystallography; biomedical equipment purchase; data collection; radiation detector; structural biology

Funds are requested for the acquisition of an area detector data collection system for macromolecular X-ray crystallography. This system would serve the needs of four structural biology groups in the Rosenstiel Basic Medical Sciences Research Center at Brandeis University. Together these groups have almost fifty people carrying out diffraction studies on biological macromolecules ranging from small proteins to intact muscle fibers. Over sixty different crystal systems are under study. The projects range in scope from de nova solution of crystal structures to study of mutant proteins generated by site-directed mutagenesis to direct observation of enzyme-substrate and -intermediate complexes by low-temperature X-ray crystallography to mapping of the entire binding surface of crystalline macromolecules by structure determination in organic solvents. These projects have significance for many areas of human health. For example, work on muscle protein components is of great importance for understanding and treating neuromuscular diseases. Mapping of binding surfaces is significant for the rational design of drugs. Studies on insulin and aldose reductase are providing crucial information for the design of more effective treatments for both Type I and Type II diabetes. These are just a few examples; nearly all of the projects are directly health-related. The equipment requested is a rotating anode generator with a high brilliance 0.3 mm focal spot, an imaging plate area detector equipped with focusing mirror optics, and cryo system to allow data collection at very low temperatures. This equipment is essential for many of the projects where the crystals are small and/or the unit cell is very large and the crystals are radiation sensitive. The requested equipment will allow us to carry out projects that are not feasible with our current facilities for data collection. Moreover, in certain cases, the availability of the new equipment will greatly facilitate the completion of projects that are now moving very slowly.

请求提供资金，用于购置区域探测器数据 用于大分子X射线晶体学的收集系统。这 系统将服务于四个结构生物学小组的需要， 布兰代斯罗森斯蒂尔基础医学科学研究中心 大学这些团体加起来有近50人， 对生物大分子的衍射研究， 小的蛋白质到完整的肌肉纤维。超过六十种不同的水晶 系统正在研究中。项目范围从de nova 晶体结构的解决方案，以研究产生的突变蛋白质 通过定点突变直接观察 酶-底物-中间体复合物低温X射线法 晶体学映射的整个结合表面的 有机晶体大分子的结构测定 溶剂.这些项目对人类的许多领域都具有重要意义。 健康例如，肌肉蛋白质成分的研究是非常重要的。 对于了解和治疗神经肌肉疾病的重要性。 结合面的测绘对于合理设计 毒品对胰岛素和醛糖还原酶的研究提供了重要的 为两种类型的更有效的治疗设计的信息 I型糖尿病和II型糖尿病。这些只是一些例子;几乎所有的 这些项目直接与健康有关。所要求的设备是 具有高亮度0.3mm焦斑的旋转阳极发生器， 成像板区域检测器，其配备有聚焦镜光学器件， 和低温系统，以允许在非常低的温度下收集数据。 该设备对于许多项目至关重要， 晶体很小和/或晶胞很大， 晶体对辐射敏感。所要求的设备将允许 我们进行的项目是不可行的与我们目前的 数据收集设施。在某些情况下， 新设备的可用性将大大促进 完成目前进展非常缓慢的项目。