STRUCTURE BIOLOGY OF ENZYMES AND DNA-BINDING PROTEINS

酶和 DNA 结合蛋白的结构生物学

• 批准号: 7369543
• 负责人: GREGORY A PETSKO
• 金额: $ 0.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369543
• 关键词: STRUCTURE; BIOLOGY; ENZYMES; DNA; BINDING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Data were collected for a variety of proteins at different stages of structure determination and exploitation. For tyrosine decarboxylase, heavy-atom derivative data were collected. This protein is a PLP-containing enzyme being studied as part of an ongoing project about protein stability and the structural basis of PLP catalysis. For chymotrypsin and aminopeptidase, ultra-high resolution data were collected as part of an ongoing project about the location of hydrogens in protein structures and the role of anisotropic motion in catalysis. For thiaminase, derivative data were collected in order to try to solve the structure of this cancer-related protein. Data were also collected on the C-terminal domain of the bacterial repressor DtxR with some putative bound peptides. A number of other crystals of projects getting underway were screened for diffraction.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。在结构测定和开发的不同阶段收集了各种蛋白质的数据。对于酪氨酸脱羧酶，收集重原子衍生物数据。这种蛋白质是一种含PLP的酶，正在研究作为一个正在进行的项目的一部分，关于蛋白质的稳定性和PLP催化的结构基础。对于胰凝乳蛋白酶和氨肽酶，超高分辨率数据被收集作为正在进行的关于蛋白质结构中氢的位置和各向异性运动在催化中的作用的项目的一部分。对于硫氨酶，收集衍生数据以试图解决这种癌症相关蛋白质的结构。还收集了细菌阻遏物DtxR的C-末端结构域与一些推定结合肽的数据。许多其他正在进行的项目的晶体被筛选衍射。