ORGANOTINS AS POSSIBLE LARVICIDES/INSECTICIDES TO CONTROL MALARIA

这项研究的主要目的是调查使用 有机锡化合物作为潜在的杀虫剂和/或杀虫剂 按蚊是这种寄生虫的主要媒介，负责 人类疟疾。开发有效的杀虫剂和/或 杀幼虫剂在努力减少传播方面将具有巨大的价值。 这种极度虚弱且常常致命的疾病。要做到这一点 客观地，有机锡化合物将被测试其有效性 防止蚊子幼虫、成年雌性蚊子和受感染的蚊子 伯氏疟原虫，一种啮齿动物特有的疟疾。杀幼虫筛查 将涉及放置蚊子幼虫和不同浓度的 有机锡在一起24小时并测定有机锡的毒性效应 有机锡化合物。将使用三种方法来确定效果 有机锡化合物对成年雌性蚊子的影响。第一种方法 涉及到将化合物局部应用于胸腔 麻醉蚊子并测定其效果。第二道工序 包括将成年蚊子放入纸箱中，这些纸箱已经过处理 感兴趣的有机锡化合物24小时。最后，它的影响 关于疟疾原生动物寄生虫发展的化合物将是 通过跟踪卵囊和子孢子期的发育来评价 伯氏疟原虫，一种啮齿动物特有的疟疾。随后，新的 将设计和合成有机锡化合物，目标是 开发更有效的化合物。 拟议的研究还旨在为学生提供培训， 哥伦比亚特区大学的研究方法 目前受雇于政府、工业和大学实验室。 为了做到这一点，学生们将参与到 项目，从有机锡化合物的合成到毒性研究 涉及蚊子幼虫和成年蚊子。

The primary objective of the research is to investigate the use of organotin compounds as potential insecticides and/or larvicides against the Anopheles mosquito, the primary vector of the parasite responsible for human malaria. The development of an effective insecticide and/or larvicide would be of tremendous value in the effort to reduce the spread of this highly debilitating and often deadly disease. To accomplish this objective, organotin compounds will be tested for their effectiveness against mosquito larvae, adult female mosquitoes and mosquitoes infected with Plasmodium berghei, a rodent specific malaria. Larvicidal screening will involve placing mosquito larvae and various concentrations of the organotins together for 24 hours and determining the toxic effects of the organotin compounds. Three methods will be used to determine the effects of the organotin compounds on adult female mosquitoes. The first method involves a topical application of the compounds to the thorax of the anesthetized mosquito and determining the effects. The second procedure involves placing adult mosquitoes into cartons that have been treated with the organotin compound of interest for 24 hours. Finally, the effects of the compounds on the malaria protozoan parasite development will be evaluated by following the development of the oocyst and sporozoite stages of Plasmodium berghei, a rodent specific malaria. Subsequently, new organotin compounds will be designed and synthesized with the aim of developing more effective compounds. The proposed research is also designed to provide training for students at the University of the District of Columbia in the research methods currently employed at government, industrial and university laboratories. To accomplish this, students will participate in all phases of the project, from the syntheses of organotin compounds to the toxicity studies involving both mosquito larvae and adult mosquito.