ORGANOTINS AS POSSIBLE LARVICIDES/INSECTICIDES TO CONTROL MALARIA

有机锡作为可能的杀幼虫剂/杀虫剂来控制疟疾

• 批准号: 6204073
• 负责人: GEORGE ENG
• 金额: $ 13.19万
• 依托单位: UNIVERSITY OF THE DISTRICT OF COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6204073
• 关键词: Anopheles; Mossbauer spectrometry; Plasmodium berghei; chemical structure function; chemical synthesis; communicable disease control; infrared spectrometry; insecticides; laboratory mouse; larva; malaria; microinjections; organic chemicals; tin; toxicant screening

The primary objective of the research is to investigate the use of organotin compounds as potential insecticides and/or larvicides against the Anopheles mosquito, the primary vector of the parasite responsible for human malaria. The development of an effective insecticide and/or larvicide would be of tremendous value in the effort to reduce the spread of this highly debilitating and often deadly disease. To accomplish this objective, organotin compounds will be tested for their effectiveness against mosquito larvae, adult female mosquitoes and mosquitoes infected with Plasmodium berghei, a rodent specific malaria. Larvicidal screening will involve placing mosquito larvae and various concentrations of the organotins together for 24 hours and determining the toxic effects of the organotin compounds. Three methods will be used to determine the effects of the organotin compounds on adult female mosquitoes. The first method involves a topical application of the compounds to the thorax of the anesthetized mosquito and determining the effects. The second procedure involves placing adult mosquitoes into cartons that have been treated with the organotin compound of interest for 24 hours. Finally, the effects of the compounds on the malaria protozoan parasite development will be evaluated by following the development of the oocyst and sporozoite stages of Plasmodium berghei, a rodent specific malaria. Subsequently, new organotin compounds will be designed and synthesized with the aim of developing more effective compounds. The proposed research is also designed to provide training for students at the University of the District of Columbia in the research methods currently employed at government, industrial and university laboratories. To accomplish this, students will participate in all phases of the project, from the syntheses of organotin compounds to the toxicity studies involving both mosquito larvae and adult mosquito.

本研究的主要目的是调查 作为潜在的杀虫剂和/或杀幼虫剂的有机锡化合物 按蚊是寄生虫的主要传播媒介， 人类疟疾 开发有效的杀虫剂和/或 杀幼虫剂在减少传播方面将具有巨大的价值 这种高度衰弱且往往致命的疾病。 为了实现这一 目的，将测试有机锡化合物的有效性 防治蚊幼虫、雌蚊成虫及受感染蚊 伯氏疟原虫一种啮齿类特有的疟疾 杀幼虫筛选 将包括放置蚊子幼虫和各种浓度的 有机锡在一起24小时，并确定的毒性作用， 有机锡化合物 将使用三种方法来确定影响 有机锡化合物对成年雌蚊的影响。 第一种方法 包括将化合物局部应用于受试者的胸部， 麻醉蚊虫并测定其效果。 第二过程 包括将成年蚊子放入经过处理的纸箱中， 目标有机锡化合物24小时。 最后，影响 这些化合物对疟疾原生动物寄生虫发育的影响， 通过跟踪卵囊和子孢子阶段的发育进行评价 伯氏疟原虫一种啮齿动物特有的疟疾 随后，新 有机锡化合物的设计和合成的目的是 开发更有效的化合物。 拟议的研究还旨在为学生提供培训， 哥伦比亚特区大学的研究方法 目前受雇于政府、工业和大学实验室。 为了实现这一目标，学生将参加所有阶段的 从有机锡化合物的合成到毒性研究 包括蚊子幼虫和成蚊。