ENZYME-MEDIATED ANALYTICAL ASSAYS

酶介导的分析测定

• 批准号: 6071448
• 负责人: Simon Bystryak
• 金额: $ 11.88万
• 依托单位: ALLIED INNOVATIVE SYSTEMS, LLC
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6071448
• 关键词: enzyme activity; enzyme linked immunosorbent assay; horseradish peroxidase; immunologic assay /test; photochemistry; technology /technique development

Development of new technology is proposed for increase the sensitivity, signal-to-noise ratio and precision of most widely used enzyme-mediated analytical assays. Except that, it can be employed for significant reducing the time of analysis and sparing costly materials. Preliminary results using model systems demonstrated the potential of the proposed technology: the amplification method allowed increasing the sensitivity of the enzyme detection 10-20-fold, compared to conventional procedures. It was also possible to reduce time of analysis 3-5-fold. Since this technology is based on the use of a general approach, it can be employed not only for immunoassays but other enzyme-mediated analytical biochemical methods. We propose to further improve and optimize the new technology, so as to generate routine technique suitable for clinical practice. We expect this development will provide less expensive method for the detection of physiologically active substances in body fluids. PROPOSED COMMERCIAL APPLICATIONS: The diagnosis of diseases is increasingly demanding the efficient and accurate quantitative determination of physiologically active substances in body fluids (see page 20 for details on market analysis). The cost of analysis using the proposed methods is supposed to be significantly smaller, compared to that for the conventional assays. The availability of advantages of our amplification method such as increase in the sensitivity, shortening the time of analysis and sparing of costly materials allow us to believe that the new products manufactured on the basis of this technology have a great commercial potential.

提出了开发新技术以提高最广泛使用的酶介导分析测定的灵敏度、信噪比和精密度。除此之外，它还可以用于显着减少分析时间和节省昂贵的材料。使用模型系统的初步结果证明了所提出的技术的潜力：与传统程序相比，扩增方法允许将酶检测的灵敏度提高10-20倍。也可以将分析时间缩短3-5倍。由于该技术是基于一般方法的使用，因此它不仅可用于免疫测定，还可用于其他酶介导的分析生化方法。我们建议进一步改进和优化新技术，以产生适合临床实践的常规技术。我们希望这一发展将提供更便宜的方法来检测体液中的生理活性物质。拟议的商业应用：疾病的诊断越来越需要对体液中的生理活性物质进行有效和准确的定量测定（有关市场分析的详细信息，请参见第20页）。与常规测定相比，使用所提出的方法的分析成本应该显著更小。我们的扩增方法的优点，如增加灵敏度，缩短分析时间和节省昂贵的材料的可用性，使我们相信，在这种技术的基础上制造的新产品具有巨大的商业潜力。