NEW METHOD FOR PROTEIN CHIPS

蛋白质芯片的新方法

• 批准号: 6072524
• 负责人: Andrzej K Drukier
• 金额: $ 10万
• 依托单位: BIOTRACES, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-18 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6072524
• 关键词: cytokine; immunologic assay /test; microarray technology; nucleic acid quantitation /detection; oligonucleotides; protein structure; proteins; technology /technique development

It is increasingly important to develop the new techniques which permit to assess bioactive molecules and to monitor the metabolic status and cellular response to external stimulation. The concurrent quantification for a large number of targets is a major advantage of the proposed super-sensitive, dedicated protein chip concept. We note, that by use of aptamers,( i.e., nucleic acid constructs), a transfer of technology from the fast developing microarrays of DNA oligonucleotides to proteins chip is accomplished. The combination of MPD instrumentation ("sensitivity") and aptamers ("specificity") may revolutionize immunodiagnostics and protein quantification. Thus, BioTraces and NeXstar have initiated a joint effort towards the improved detection and quantitation of proteins, viruses and bacteria. The goal of this project is to test MPD/Aptamer enhanced methods for detection of cytokine targets and compare their performance with prior-art techniques. PROPOSED COMMERCIAL APPLICATIONS: The wide range of applications for MPD includes immunoassays and RNA/DNA analysis. Using MPD enhanced techniques, traces of biological molecules can be quantified at much lower concentrations. Some of the most important applications of MPD are in immunoassays, quantitative PCR and direct detection of DNA at the few copies level. These markets are predicted to be about $5 billion/year by 2000. MPD may account for a few percent of this market.

开发新的技术来评估生物活性分子并监测代谢状态和细胞对外部刺激的反应越来越重要。大量目标的同时定量是所提出的超灵敏、专用蛋白质芯片概念的主要优点。我们注意到，通过使用适体，（即，核酸构建体），完成了从快速发展的DNA寡核苷酸微阵列到蛋白质芯片的技术转移。MPD仪器（“灵敏度”）和适体（“特异性”）的组合可以彻底改变免疫诊断和蛋白质定量。因此，BioTraces和NeXstar已经开始共同努力改进蛋白质、病毒和细菌的检测和定量。该项目的目标是测试MPD/适体增强的方法用于检测细胞因子靶标，并将其性能与现有技术进行比较。建议的商业应用：MPD的广泛应用包括免疫测定和RNA/DNA分析。使用MPD增强技术，可以在低得多的浓度下定量痕量的生物分子。MPD的一些最重要的应用是在免疫测定、定量PCR和在几个拷贝水平上直接检测DNA。据预测，到2000年，这些市场将达到每年约50亿美元。MPD可能占这个市场的百分之几。