Dual MPD Imager

双 MPD 成像仪

• 批准号: 6737738
• 负责人: Andrzej K Drukier
• 金额: $ 10万
• 依托单位: BIOTRACES, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6737738
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; imaging /visualization /scanning; iodine; particle counter; proteomics; radionuclides; thin layer chromatography; two dimensional gel electrophoresis

DESCRIPTION (provided by applicant): Many biochemical operations can be facilitated by differentially labeling two samples that differ in only one parameter, performing biochemical separations on the mixture and afterward determining the amount of each label in each separated spot. Such measurements are easiest to perform and most accurate when both isotopes are measured concurrently rather than sequentially. Recent work has shown the utility of this approach to determine proteins that are more or less abundant in cancerous tissue compared to normal. We plan to develop a Dual MPD Imager to measure the planar distribution of two isotopes of iodine (125-I and 131-I) in samples such as 2D gels, TLC plates, protein chips, etc. The dual imager will separately measure the amount of each isotope in a single scan. We expect to have less than 1% cross talk between the two labels and better than 0.5 mm spatial resolution. Preliminary results show an excellent separation of signals from the two isotopes.

描述（由申请人提供）： 许多生物化学操作可以通过差异标记两个仅在一个参数上不同的样品，对混合物进行生物化学分离，然后确定每个分离点中每个标记的量来促进。当两种同位素同时测量而不是顺序测量时，这种测量最容易执行并且最准确。最近的工作表明，这种方法可以用来确定与正常组织相比，癌组织中或多或少丰富的蛋白质。 我们计划开发一种双MPD成像仪，用于测量样品中碘的两种同位素（125-I和131-I）的平面分布，如2D凝胶，TLC板，蛋白质芯片等。双成像仪将在单次扫描中分别测量每种同位素的量。我们希望两个标签之间的串扰小于1%，空间分辨率优于0.5 mm。 初步结果表明，两种同位素的信号分离良好。