Proteomic analysis during stem cell differentiation

描述(申请者逐字描述)：干细胞被定义为细胞 具有自我更新和差异化的能力。茎的这一特性 细胞在发育过程中支持生长和分化，并维持 整个成年生活中的动态平衡和修复过程。身份的鉴定 驱动多潜能干细胞生长的新蛋白质或指导 他们的分化和血统承诺，具有根本意义 了解干细胞生物学及其医学应用。中环 对这些蛋白质的鉴定和特性是 一种研究蛋白质表达的超灵敏方法的可行性 这些细胞在各种条件下的分布情况非常敏感， 量化、高效的时尚。唉，现有技术的技术 蛋白质差异显示不够灵敏及其动态 检测范围不足以研究低丰度蛋白质。BIOTRACES 开发了专有的多光子探测(MPD)技术。使用MPD 技术，我们正在开发一个集成的蛋白质组系统，包括MPD 蛋白质差异显示成像仪，至少100倍 比其他技术更灵敏。此第一阶段提案的目的是 应用基于MPD的蛋白质组学工具来识别和表征 在造血干细胞过程中的差异修饰或表达 世系承诺和分化。 建议的商业应用： MPD已成功地应用于RNA/DNA分析、免疫分析和蛋白质组学。 MPD增强的蛋白质分析，包括更好的细胞因子免疫分析将 实现更好的生物医学诊断和新的治疗模式。这是一个大型的，多个- 十亿美元/年的市场。BioTraces可能会成为这个市场的一部分主导者 年收入估计在1亿至2亿美元之间。我们正在开发 MPD使超灵敏的蛋白质芯片成为可能，包括干细胞的P芯片。这些新的 技术允许我们捕获相当一部分诊断蛋白质组学和 诊断肿瘤学市场。

DESCRIPTION (Applicant's Description Verbatim): Stem cells are defined as cells with the ability both to self renew and to differentiate. This property of stem cells underpins growth and differentiation during development and sustains homeostasis and repair processes throughout adult life. The identification of novel proteins that drive pluriopotent stem cell growth or factors that direct their differentiation and lineage commitment, are of fundamental significance in the understanding of stem cell biology and its medical applications. Central to the identification and characterization of these proteins is the availability of a super sensitive method for studying the protein expression profile of these cells under a wide range of conditions in a sensitive, quantitative, and efficient fashion. Alas, the prior-art techniques of differential display of proteins arc not sensitive enough and their dynamic detection range is not adequate for studying low abundance proteins. BIOTRACES has developed a proprietary Multi Photon Detection (MPD) technology. Using MPD technology, we are developing an integrated proteomic system including the MPD Imager for differential display of protein that is at least one hundred times more sensitive than other techniques. The purpose of this Phase I proposal is to apply MPD-based proteomics tools to identify and characterize proteins that are differentially modified or expressed during hematopoietic stem cells lineage commitment and differentiation. PROPOSED COMMERCIAL APPLICATION: MPD has successfully been applied to RNA/DNA analysis, immunoassays and proteomics. The MPD-enhanced analysis of proteins, including better immunoassays for cytokines will enable better biomedical diagnostics and new therapeutic modalities. This is a large, multi- billion dollar/yr market. BioTraces may become a dominant player in part of this market with income estimated to be in the range of 100-200 million dollars annually. We are developing MPD enabled ultrasensitive protein-chips, including the P-chip for stem cells. These new techniques permit us to capture a considerable segment of diagnostic proteomics and diagnostic oncology market.