Proteomic analysis during stem cell differentiation

描述（申请人逐字描述）：干细胞被定义为细胞 具有自我更新和分化的能力。茎的这种性质 细胞在发育过程中支持生长和分化， 在整个成年生活中的自我平衡和修复过程。的识别 驱动多能干细胞生长的新蛋白质或指导 他们的分化和血统承诺，具有根本意义， 对干细胞生物学及其医学应用的理解。中央 鉴定和表征这些蛋白质的关键是 一种超灵敏的蛋白质表达研究方法的可用性 这些细胞的分布在广泛的条件下， 定量和高效的方式。可惜的是， 蛋白质的差异显示不够灵敏， 检测范围不足以研究低丰度蛋白质。生物痕迹 开发了一种专有的多光子检测（MPD）技术。使用MPD 技术，我们正在开发一个集成的蛋白质组学系统，包括MPD 一种用于蛋白质差异显示的成像仪， 比其他技术更敏感。第一阶段提案的目的是 应用基于MPD的蛋白质组学工具来识别和表征蛋白质， 在造血干细胞分化过程中， 血统承诺和差异化。 拟定商业应用： MPD已成功应用于RNA/DNA分析、免疫分析和蛋白质组学。 MPD增强的蛋白质分析，包括更好的细胞因子免疫测定， 使更好的生物医学诊断和新的治疗方式。 这是一个大的，多- 亿美元/年的市场。 BioTraces可能会成为这一市场的主导者， 据估计，这一年的收入将在1亿至2亿美元之间。 我们正在开发 MPD使超灵敏蛋白芯片成为可能，包括用于干细胞的P芯片。 这些新 技术使我们能够捕获诊断蛋白质组学的相当大的部分， 肿瘤诊断市场

DESCRIPTION (Applicant's Description Verbatim): Stem cells are defined as cells with the ability both to self renew and to differentiate. This property of stem cells underpins growth and differentiation during development and sustains homeostasis and repair processes throughout adult life. The identification of novel proteins that drive pluriopotent stem cell growth or factors that direct their differentiation and lineage commitment, are of fundamental significance in the understanding of stem cell biology and its medical applications. Central to the identification and characterization of these proteins is the availability of a super sensitive method for studying the protein expression profile of these cells under a wide range of conditions in a sensitive, quantitative, and efficient fashion. Alas, the prior-art techniques of differential display of proteins arc not sensitive enough and their dynamic detection range is not adequate for studying low abundance proteins. BIOTRACES has developed a proprietary Multi Photon Detection (MPD) technology. Using MPD technology, we are developing an integrated proteomic system including the MPD Imager for differential display of protein that is at least one hundred times more sensitive than other techniques. The purpose of this Phase I proposal is to apply MPD-based proteomics tools to identify and characterize proteins that are differentially modified or expressed during hematopoietic stem cells lineage commitment and differentiation. PROPOSED COMMERCIAL APPLICATION: MPD has successfully been applied to RNA/DNA analysis, immunoassays and proteomics. The MPD-enhanced analysis of proteins, including better immunoassays for cytokines will enable better biomedical diagnostics and new therapeutic modalities. This is a large, multi- billion dollar/yr market. BioTraces may become a dominant player in part of this market with income estimated to be in the range of 100-200 million dollars annually. We are developing MPD enabled ultrasensitive protein-chips, including the P-chip for stem cells. These new techniques permit us to capture a considerable segment of diagnostic proteomics and diagnostic oncology market.