GLASS BASED BIOCHIP AND OPTICAL DETECTOR

新维度研究与仪器（NDR）提出了一种基于玻璃的生物芯片系统，这是一种独特的平台，用于获取、分析和管理复杂的遗传信息。这种创新的玻璃基芯片及其相关的芯片读取器能够实时监测DNA杂交，通过使用分子信标染料和矩阵寻址电场来精确控制化学反应。基于玻璃的生物芯片采用自寻址、自组装的微电子系统，可以在微观格式下主动执行和控制多步骤和多重反应。通过使用我们的分子信标，只有完全匹配的DNA对才会发出荧光，（不匹配的DNA对不会发出荧光），我们可以实时监测杂交过程。此外，杂交过程中的化学洗涤将被消除。我们将展示和开发一种检测低拷贝数核酸靶标的方法和设备，该方法和设备结合1)选择性杂交的高特异性分子信标，2)荧光探针附近的高灵敏度检测器，3)选择性杂交的电场。拟议的商业应用：生物芯片技术使研究人员和临床医生能够比以往任何时候都更快、更有效地分析大量的遗传信息，从而精确地确定感染、非感染和遗传疾病。该系统已被开发为一种工具，以帮助了解人类基因组，并改善疾病的诊断、监测和治疗，如癌症、结核病、艾滋病毒、HB等。

New Dimension Research and Instrument (NDR), proposes to make a glass-based BioChip system, a unique platform for acquiring, analyzing and managing complex genetic information. This innovative glass-based chip, and its associated chip reader are able to real- time monitor DNA hybridization by using molecular beacon dye and a matrix-addressable electrical field for precise control of chemical reactions. The glass-based BioChip uses a self-addressable, self- assembling micro-electronic system, which can actively carry out and control multi-step and multiplex reactions in microscopic formats. By using our molecular beacon in which only exactly matched DNA pairs will be fluorescent, (unmatched pairs will not fluoresce), we can real-time monitor the hybridization process. Moreover, the chemical washing in the hybridization process will be eliminated. We will demonstrate and develop a method and a device for detecting low copy numbers of nucleic acid target by combining 1) high specificity of molecular beacon for selective hybridization, 2) high sensitivity detector near the fluorescent probe, and 3) electric field for selectivity hybridization. PROPOSED COMMERCIAL APPLICATION: BioChip technology enables researchers and clinicians to analyze large amounts of genetic information more rapidly and efficiently than was ever before possible, which can precisely determine infection, non-infection, and genetic diseases. The system has been developed as a tool to help understand the human genome and to improve the diagnosis, monitoring and treatment of diseases, such as cancers, TB, HIV, HB, etc.