RESOURCE FOR ANALYSIS OF YEAST FUNCTIONAL GENOMICS

酵母功能基因组学分析资源

• 批准号: 2870341
• 负责人: JAMES I GARRELS
• 金额: $ 9.93万
• 依托单位: PROTEOME, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 1999-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2870341
• 关键词: Saccharomyces cerevisiae; computer program /software; computer system design /evaluation; data management; fungal genetics; fungal proteins; genome; informatics; molecular biology information system; nucleic acid sequence; protein structure function; yeast two hybrid system

This proposal is to create a Yeast Functional Genomics Resource (YFGR[TM]) to manage, present and analyze the explosion of functional genomic data for Saccharomyces cerevisiae. YFGR will encompass new presentation and analysis tools with links into the successfully developed Yeast Protein Database (YPD[TM]). YPD provides over 50,000 annotation lines of scientific results, curated from more than 7500 publications. In this post-genomic phase of yeast research, technology has shifted to genome- wide, large-scale functional analyses, which include DNA microarrays, SAGE experiments, systematic gene knockouts, and systematic two-hybrid analyses. The enormous amount of data urgently requires organization and integration with other database resources to be more easily and completely evaluated by academic and industrial researchers. The data sets will be reformatted in YFGR as relational database tables and annotated with experimental details. Software will be developed to present expression profile data and protein interaction network data. The analysis software will use YPD to find common properties for co-regulated proteins or proteins that show two-hybrid interaction, giving the user unparalleled power to connect functional genomic data with the prior knowledge of yeast biology. PROPOSED COMMERCIAL APPLICATIONS: The Yeast Protein Database (YPD[TM]) is used by biotechnology, pharmaceutical and bioinformatics companies, in the context of anti-fungal research, comparative genomics, and increasingly to interpret functional genomics. Current bioinformatics tools allow sequence analysis, but few software tools currently exist for functional analyses. The Yeast Functional Genomics Resource (YFGR[TM]) will enable companies to realize more value from their investments in functional genomic technologies. This suite of tools developed for the YFGR will be applicable for use with Proteome Databases for other model organism beina developed by Proteome, Inc.

该提案旨在创建一个酵母功能基因组学资源（YFGR[TM]），以管理、呈现和分析酿酒酵母功能基因组数据的爆炸。YFGR将包括新的展示和分析工具，并与成功开发的酵母蛋白质数据库（YPD[TM]）相链接。YPD提供了超过50，000条科学成果注释，来自7500多份出版物。在酵母研究的后基因组阶段，技术已经转向全基因组、大规模功能分析，包括DNA微阵列、SAGE实验、系统基因敲除和系统双杂交分析。大量的数据迫切需要组织和与其他数据库资源的集成，以便学术和工业研究人员更容易和完整地评估。数据集将在YFGR中重新格式化为关系数据库表，并注明实验细节。将开发软件来呈现表达谱数据和蛋白质相互作用网络数据。该分析软件将使用YPD找到共调节蛋白质或显示双杂交相互作用的蛋白质的共同特性，为用户提供无与伦比的能力，将功能基因组数据与酵母生物学的先验知识联系起来。拟议的商业应用：酵母蛋白质数据库（YPD[TM]）被生物技术、制药和生物信息学公司用于抗真菌研究、比较基因组学，并越来越多地用于解释功能基因组学。目前的生物信息学工具允许序列分析，但目前很少有软件工具存在的功能分析。酵母功能基因组学资源（YFGR[TM]）将使公司能够从他们对功能基因组技术的投资中实现更多价值。为YFGR开发的这套工具将适用于与Proteome，Inc.开发的其他模式生物的蛋白质组数据库一起使用。