PROTEOME DATABASE FOR PATHOGENIC FUNGI CANDIDA ALBICANS

致病真菌白色念珠菌蛋白质组数据库

• 批准号: 2712413
• 负责人: JAMES I GARRELS
• 金额: $ 10万
• 依托单位: PROTEOME, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2712413
• 关键词: Candida albicans; Internet; biomedical resource; chemical information system; computer assisted sequence analysis; computer program /software; computer system design /evaluation; fungal genetics; gel electrophoresis; genome; protein sequence; protein structure

This project seeks to build a proteome database for the pathogenic fungi Candida albicans as a companion database to the already successfully developed and commercialized Yeast Protein Database (YPD(TM)) for the model organism Saccharomyces cerevisiae. This database will combine curated proteome data will protein expression profiles (2D gel electrophoresis data), and it will have extensive links to YDP(TM) based on sequence homologies. The Candida albicans proteome database (CalPD(TM)) will consist of a series of Protein Reports, each of which is a Web page reporting properties and free-text annotations for one protein. The methods of curation from the literature and review of all material by editors will be similar to the methods used for the development of YPD(TM). This project will also use two-dimensional gel electrophoresis, a technique of renewed importance for functional genome analysis, to develop a master protein map for C. albicans with identification of major proteins. The curated database and the C. albicans protein map will be used in Phase II to develop a full protein expression profile database designed to serve the needs of the pharmaceutical industry which is engaged in extensive research to find new drug targets for the important human pathogen, Candida albicans. PROPOSED COMMERCIAL APPLICATION: With entire genome sequences becoming available, it becomes possible to describe the proteome, or the set of proteins coded by the genome. This research will help to develop a proteome database for the important pathogenic fungi Candida albicans. The database will contain the accumulated knowledge for the C. albicans proteins from literature curation, and protein expression profiling data for the C. albicans proteome provided by the parallel technique of 2D electrophoresis. The database will link to the commercially successful Yeast Protein Database (YPD"). Proteome data is in demand in industry to extend genomic data for antifungal research and for comparative genomics, and for direct annotation of human proteins with yeast homologues.

本项目旨在建立病原真菌的蛋白质组数据库 白色念珠菌作为一个伴侣数据库， 开发和商业化的酵母蛋白质数据库（YPD（TM））， 模式生物酿酒酵母。这个数据库将联合收割机 精选的蛋白质组数据将蛋白质表达谱（2D凝胶 电泳数据），它将有广泛的联系，以YDP（TM）为基础 关于序列同源性。白色念珠菌蛋白质组数据库（CalPD（TM）） 将由一系列蛋白质报告组成，每个报告都是一个网页 报告一种蛋白质的属性和自由文本注释。的 从文献和审查所有材料的策展方法， 编辑器将类似于用于开发的方法 YPD（TM）。该项目还将使用二维凝胶电泳， 一种对功能基因组分析重新具有重要意义的技术， 绘制C.白色念珠菌，鉴定为主要 proteins.策展数据库和C.白色念珠菌蛋白质图谱将 用于第二阶段开发完整的蛋白质表达谱数据库 旨在满足制药行业的需求， 从事广泛的研究，以寻找新的药物靶点的重要 人类病原体白色念珠菌 拟定商业应用： 随着整个基因组序列的可用， 描述蛋白质组，或由基因组编码的一组蛋白质。这 研究将有助于开发一个蛋白质组数据库， 致病真菌白色念珠菌数据库将包含 为C.文献中的白色念珠菌蛋白 策展和C.白色 蛋白质组由2D电泳的平行技术提供。的 数据库将链接到商业上成功的酵母蛋白质数据库 (YPD").蛋白质组数据在工业上是需要的，以扩展基因组数据， 抗真菌研究和比较基因组学，以及直接 用酵母同源物注释人类蛋白质。