LOW COST SCREENING TOOL FOR HOLLOW FIBER BIOREACTOR RAND

适用于中空纤维生物反应器 RAND 的低成本筛选工具

• 批准号: 2536524
• 负责人: MICHAEL J GRAMER
• 金额: $ 10万
• 依托单位: BIOVEST INTERNATIONAL, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2536524
• 关键词: biomedical equipment development; bioreactors; cell line; tissue /cell culture

Hollow-fiber bioreactor technology is an economical alternative to traditional cell culture methods for the production of cells and cell- derived products, and is finding increased use for novel applications in the fields of tissue engineering, gene therapy, and pharmacology. Hollow-fiber bioreactors are also reducing the suffering of animals by serving as logical replacements for ascites production methods. However, a major drawback of hollow-fiber technology is an inherent lack of predictability; there is no efficient screening method to determine how well a new cell line will perform in a hollow-fiber system, or how well an established cell line will perform under new conditions. To address this problem, a small, inexpensive, and easy to use screening tool has been developed at CELLEX BIOSCIENCES. This tool incorporates the same fibers as in a production system, but requires no pumping for oxygenation or medium delivery so that dozens of conditions can be tested simultaneously in a single incubator. This research will be used to test the validity of this micro hollow-fiber screening tool as a predictor of cell line performance in large scale hollow fiber systems. This tool has the potential of substantially increasing the efficiency and reliability of hollow-fiber systems, much as screening tools such as 96-well plates have done for stirred tank systems. PROPOSED COMMERCIAL APPLICATION Most importantly, a low cost screening tool will be developed and validated to fill a current market need. CELLEX could also use this tool along with other information learned in this research to provide a battery of screening tests as an optimization service. In addition, a new medium formulation specifically optimized for hollow-fiber bioreactors could be developed in this research.

中空纤维生物反应器技术是一种经济的替代方案 用于生产细胞和细胞的传统细胞培养方法 衍生产品，并且正在越来越多地用于新颖的应用 在组织工程、基因治疗和药理学领域。 中空纤维生物反应器还通过以下方式减少动物的痛苦 作为腹水生产方法的合理替代品。 然而，中空纤维技术的一个主要缺点是固有的缺乏 可预测性；没有有效的筛选方法来确定 新细胞系在中空纤维系统中的表现如何，或者如何 一个已建立的细胞系将在新条件下表现良好。 到 解决这个问题，一种小型、廉价且易于使用的筛选 该工具是由 CELLEX BIOSCIENCES 开发的。 该工具包含 与生产系统中的纤维相同，但不需要泵送 充氧或介质输送，以便可以处理数十种情况 在单个培养箱中同时进行测试。 这项研究将用于 测试这种微型中空纤维筛选工具作为筛选工具的有效性 大规模中空纤维系统中细胞系性能的预测器。 该工具具有显着提高效率的潜力 和中空纤维系统的可靠性，就像筛选工具一样 正如 96 孔板在搅拌槽系统中所做的那样。 拟议的商业应用 最重要的是，将开发一种低成本筛选工具 经验证可以满足当前的市场需求。 CELLEX 也可以使用这个 工具以及本研究中学到的其他信息，以提供 一系列筛选测试作为优化服务。 此外， 专门针对中空纤维优化的新介质配方 这项研究可以开发生物反应器。