LOW COST SCREENING TOOL FOR HOLLOW FIBER BIOREACTOR RAND

中空纤维生物反应器技术是一种经济的替代 用于生产细胞的传统细胞培养方法和细胞- 衍生产品，并正在发现更多的新应用 在组织工程、基因治疗和药理学领域。 中空纤维生物反应器还通过以下方式减少动物的痛苦 作为腹水生产方法的合理替代品。 然而，中空纤维技术的一个主要缺点是固有的缺陷。 可预测性；没有有效的筛选方法来确定 新的细胞系在中空纤维系统中的表现如何，或者如何 一个已建立的细胞系将在新的条件下表现良好。至 针对这一问题，提出了一种体积小、价格便宜、使用方便的筛分方法 该工具已在CELLEX生物科学公司开发。该工具结合了 与生产系统中的纤维相同，但不需要泵送 充氧或介质输送，以便数十种条件可以 在单个孵化器中同时进行测试。这项研究将用于 为了测试这种微型中空纤维筛分工具作为一种 大规模中空纤维系统中细胞系性能的预报器。 这种工具有可能大大提高效率。 以及中空纤维系统的可靠性，就像筛选工具一样 就像96孔板对搅拌槽系统所做的那样。 建议的商业应用 最重要的是，将开发一种低成本的筛查工具， 经过验证，可以满足当前的市场需求。塞莱克斯也可以用这个 工具以及在本研究中了解到的其他信息来提供 一系列筛选测试作为优化服务。此外, 一种专门针对中空纤维进行优化的新型介质配方 在这项研究中，可以开发生物反应器。

Hollow-fiber bioreactor technology is an economical alternative to traditional cell culture methods for the production of cells and cell- derived products, and is finding increased use for novel applications in the fields of tissue engineering, gene therapy, and pharmacology. Hollow-fiber bioreactors are also reducing the suffering of animals by serving as logical replacements for ascites production methods. However, a major drawback of hollow-fiber technology is an inherent lack of predictability; there is no efficient screening method to determine how well a new cell line will perform in a hollow-fiber system, or how well an established cell line will perform under new conditions. To address this problem, a small, inexpensive, and easy to use screening tool has been developed at CELLEX BIOSCIENCES. This tool incorporates the same fibers as in a production system, but requires no pumping for oxygenation or medium delivery so that dozens of conditions can be tested simultaneously in a single incubator. This research will be used to test the validity of this micro hollow-fiber screening tool as a predictor of cell line performance in large scale hollow fiber systems. This tool has the potential of substantially increasing the efficiency and reliability of hollow-fiber systems, much as screening tools such as 96-well plates have done for stirred tank systems. PROPOSED COMMERCIAL APPLICATION Most importantly, a low cost screening tool will be developed and validated to fill a current market need. CELLEX could also use this tool along with other information learned in this research to provide a battery of screening tests as an optimization service. In addition, a new medium formulation specifically optimized for hollow-fiber bioreactors could be developed in this research.