GENETIC MODEL OF ENDURANCE EXERCISE IN RATS

描述（改编自申请人的摘要）： 这项工作是为了确定基因决定之间的差异低， 哺乳动物的高耐力表现。 第一步，正如这里所介绍的， 是培育出不同品系的老鼠， 通过选择性育种获得的耐力跑。 两项初步研究确定 低耐力和高耐力磨合的选择育种参数 大鼠 首先，跑步机耐力跑的遗传性是在一个 Sprague-Dawley大鼠的小测试群体（n=48）。 这段距离一直跑到 疲劳被用来衡量耐力。 两对最高的， 最低的是选择性繁殖的。 高和低选择的后代 老鼠在跑步方面表现出29.4%的差异， 狭义遗传力（h2）为0.50。 第二，测量192 N/Nih大鼠的耐力跑步性能， 选择性繁殖的目的是在性能的极端配对。 的 选择N/Nih是因为它们具有广泛的遗传异质性。 十三 在性能的极端情况下选择交配对， 宽选择差。 13个高选择的女性平均跑 3.55比13个低选择的女性多一倍。 第13章被选中 雄性比低雄性跑远5.59倍。 这些数据预测， 大鼠跑步耐力性能的育种价值。 具体目标 本工作的目的是：1. 创造低耐力和高耐力的菌株 通过N/Nih大鼠的选择性繁殖直至遗传， 高原 2. 继续进行选育试验的规模较小 Sprague-Dawley大鼠群体直至遗传平台期。 鉴定负责低和高之间差异的基因 运动耐力将为理解遗传学奠定更广泛的基础。 健康和疾病的起源。 例如，低性能选择大鼠 可能集中了决定心力衰竭发展的基因， 寿命缩短。 此外，这些运动表现模型可以 作为拟议的“生理组项目”的核心组成部分， 功能基因组学

DESCRIPTION (Adapted from the applicant's abstract): The long term goal of this work is to identify genes that dictate the difference between low and high endurance performance in mammals. The first step, as presented here, is to develop divergent strains of rats that express to the extremes of endurance running by selective breeding. Two preliminary studies defined parameters for selective breeding for low and high endurance running in rats. First, heritability of treadmill endurance running was estimated in a small test population (n=48) of Sprague-Dawley rats. The distance run until exhaustion was used as a measure of endurance. Two pairs of the highest and lowest were selectively bred. The offspring of the high and low selected rats demonstrated a 29.4 percent difference in running performance in the first generation and had a narrow sense of heritability (h2) of 0.50. Second, the endurance running performance of 192 N/Nih rats was measured for the purpose of selective breeding pairs at the extremes of performance. The N/Nih were chosen because they have wide genetic heterogeneity. Thirteen mating pairs were selected at the extremes of performance and demonstrated a wide selection differential. The 13 high selected females ran on average 3.55 times further than the 13 low selected females. The 13 high selected males ran 5.59 times further than the low males. These data predict a high breeding value for running endurance performance in rats. The specific aims of this work are to: 1. Create strains of low and high endurance performance rats by selective breeding of the N/Nih rats until inheritance plateaus. 2. Continue selection and breeding of the smaller test population of Sprague-Dawley rats until inheritance plateaus. Identification of genes responsible for the differences between low and high exercise endurance would form a broader base for understanding the genetic origins of health and disease. For example, low performance selected rats may concentrate genes that determine the development of heart failure or reduced longevity. In addition, these models of exercise performance could serve as a central component of the proposed "Physiome Project" for functional genomics.