SORTING AND ASSEMBLY OF THE HSV-1 TEGUMENT PROTEIN VHS

HSV-1 外皮蛋白 VHS 的分类和组装

• 批准号: 2810598
• 负责人: DUNCAN W. WILSON
• 金额: $ 15.12万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2810598
• 关键词: Golgi apparatus; cell line; cell membrane; chimeric proteins; confocal scanning microscopy; green fluorescent proteins; herpes simplex virus 1; immunocytochemistry; immunoelectron microscopy; immunoprecipitation; intracellular transport; laboratory rabbit; organelles; protein signal sequence; protein structure function; site directed mutagenesis; virion; virus assembly; virus envelope; virus protein; western blottings

The Herpes viruses are responsible for a large number of human diseases. All Herpes viruses possess a proteinaceous layer termed tegument which lines the inner surface of the viral envelope. Correct assembly of tegument and envelope is essential for the production of an infectious virus particle, and thus for the progression of disease. However, little is known of how tegument proteins recognize and bind to specific cellular membranes, nor how they ensure their assembly into the maturing virion. This proposal will investigate membrane and tegument sorting information present within the Herpes simplex virus (HSV) tegument protein vhs. We will identify the cytoplasmic organelle which binds vhs, and determine the role of this association in the HSV life cycle. Sequences responsible for vhs/organelle binding will be identified, and we will determine which regions of vhs ensure incorporation of this protein into the mature virus. Information obtained from this study will help in the design of agents able to interfere with vhs assembly into the HSV particle, reducing the virulence of this serious human pathogen.

疱疹病毒是人类许多疾病的罪魁祸首。 所有的疱疹病毒都有一层称为被膜的蛋白质层 在病毒包膜的内表面划上一条线。正确组装 外皮和信封是生产传染性支气管炎的关键 病毒颗粒，从而促进疾病的发展。然而， 关于被膜蛋白是如何识别和结合特定基因的知之甚少 细胞膜，也不知道它们如何确保它们组装成成熟的 病毒粒子。 该提案将调查薄膜和被膜的分类信息 存在于单纯疱疹病毒(HSV)被膜蛋白VHS内。我们 将鉴定与VHS结合的细胞质细胞器，并确定 这种关联在HSV生命周期中的作用。数列 将确定负责VHS/细胞器绑定的人员，我们将 确定VHS的哪些区域确保将该蛋白质整合到 成熟的病毒。从这项研究中获得的信息将有助于 能够干扰VHS组装成HSV的代理的设计 颗粒，降低这种严重的人类病原体的毒力。