ASSEMBLY AND FUNCTION OF THE HERPES SIMPLEX ENVELOPE

单纯疱疹病毒包膜的组装和功能

• 批准号: 6259315
• 负责人: DUNCAN W. WILSON
• 金额: $ 28.66万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-03-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6259315
• 关键词: capsid; green fluorescent proteins; herpes simplex virus 1; host organism interaction; immunocytochemistry; intracellular transport; laboratory rabbit; protein transport; temperature sensitive mutant; tissue /cell culture; virus assembly; virus envelope; virus infection mechanism; virus protein

Formation of the herpes simplex virus (HSV) envelope, and egress of enveloped virions from cells, is critical for the production of infectious particles and the spread of disease. Unfortunately, little is know of how HSV capsids and tegument proteins refashion cell membranes into envelopes. Similarly, the identity of the cytoplasmic organelles utilized for viral exocytosis remains obscure. Our working model is that HSV capsids acquire their final envelope in the cell cytoplasm by attaching to the surface of the Golgi apparatus or endosomes, then budding into them. Using novel assay techniques developed in this laboratory, we will purify then establish the identity and function of cytoplasmic organelles utilized for HSV envelopment. The mechanism of association of capsids and tegument proteins with the surface of these organelles, the first step in viral envelopment, will then be determined. Finally, the interaction of capsids with cytoplasmic compartments will be visualized in living cells by imaging the trafficking of Green Fluorescent Protein tagged capsids during a single, synchronized wave of assembly. Information obtained from this study will help in the design of agents able to interfere with HSV envelopment, thus reducing the virulence of this serious human pathogen.

单纯疱疹病毒(HSV)包膜的形成和出口 从细胞中分离出被包裹的病毒粒子，对于产生感染性 颗粒和疾病的传播。不幸的是，人们对HSV是如何 衣壳和被膜蛋白将细胞膜重新包装成包膜。 同样，用于病毒的细胞质细胞器的特性 胞吐作用仍不清楚。我们的工作模型是HSV衣壳病毒获得他们的 通过附着在高尔基体表面的细胞质中的最终包膜 器官或内体，然后发芽进入它们。使用新的检测技术 在这个实验室中开发的，我们将净化然后建立身份和 胞质细胞器对单纯疱疹病毒包膜的作用。这一机制 衣壳和被膜蛋白与这些细胞表面的结合 细胞器是病毒包膜的第一步，然后将被确定。 最后，衣壳与细胞质隔间的相互作用将是 通过成像绿色荧光在活细胞中的传输 蛋白质标记衣壳在单一的，同步的组装波。 从这项研究中获得的信息将有助于设计能够 干扰HSV的包膜，从而降低这种严重的毒力 人类病原体。