Trafficking of Proteins to the Herpes Simplex Tegument

蛋白质运输到单纯疱疹皮膜

• 批准号: 7682789
• 负责人: DUNCAN W. WILSON
• 金额: $ 58.06万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-15 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7682789
• 关键词: Address; Amino Acids; Automobile Driving; Binding; Biochemical; Biochemistry; Capsid; Cell membrane; Cells; Cellular Membrane; Complex; Cytoplasm; Cytoplasmic Tail; Disease; Glycoproteins; Green Fluorescent Proteins; Herpes Simplex Infections; Herpesviridae; In Vitro; Intracellular Membranes; Left; Lipids; Mass Spectrum Analysis; Mediating; Membrane; Membrane Protein Traffic; Methodology; Modeling; Molecular; Nature; Nuclear; Nuclear Inner Membrane; Pathway interactions; Process; Property; Proteins; Protocols documentation; Reporter; Role; Scaffolding Protein; Simplexvirus; Sorting - Cell Movement; Structure; Surface; Tail; Techniques; Testing; Virion; Virus Assembly; Western Blotting; env Gene Products; intracellular protein transport; neurovirulence; particle; protein transport; research study

All Herpes viruses possess a proteinaceous layer termed tegument which lines the inner surface of their envelope. Tegument is acquired both at the inner nuclear membrane during primary envelopment, and in the cytoplasm during secondary envelopment. These tegument layers are morphologically distinct, however, little is known of how their molecular compositions differ. Furthermore, the mechanisms by which tegument proteins become bound to membranes and incorporated into the assembling HSV particle are poorly understood. We have identified a 42 amino acid membrane targeting motif in the amino terminus of vhs, a tegument component that is an important neurovirulence determinant. This membrane sorting motif is also sufficient to direct a GFP reporter into the HSV particle. Furthermore, we have shown that vhs binds to the cytoplasmic tail of the envelope glycoprotein gH. The aims of this proposal dissect the molecular details of membrane targeting by the amino terminus of vhs, tests the role of gH in this process and examines the nature of vhs in the perinuclear HSV particle. We also use newly developed methodologies to investigate the general composition and function of the perinuclear HSV particle, examine targeting of vhs to the perinuclear virion and compare the envelope and tegument of these poorly characterized particles with those of mature virions.

所有疱疹病毒都有一层称为被皮的蛋白质层，它排列在内表面