Biochemistry of Herpes Simplex Tegument Components

单纯疱疹外皮成分的生物化学

• 批准号: 7090988
• 负责人: DUNCAN W. WILSON
• 金额: $ 28.89万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2007-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7090988
• 关键词: Alphaherpesvirinae; Herpes simplex disease; biochemistry; host organism interaction; microorganism culture; protein binding; protein purification; protein structure function; virion; virus assembly; virus envelope; virus protein

The Herpes viruses are responsible for many human diseases. All Herpes viruses possess a proteinaceous layer termed tegument which lines the inner surface of their envelope. Correct assembly of tegument and envelope is essential for the production of an infectious viral particle, and thus for the progression of disease. However, little is known of how tegument proteins recognize and bind to specific cellular membranes, nor how they ensure their assembly into the maturing virion. The first part of this proposal will investigate the biochemistry of the Herpes simplex virus (HSV) protein vhs, a component of the HSV tegument. We have previously identified several biochemically distinct forms of vhs in infected cells. We will now investigate the relationship between these molecules and determine their importance for assembly of vhs into tegument. Next, we will dissect the molecular mechanism by which vhs binds to intracellular membranes. Finally, we have successfully isolated primary enveloped HSV particles from the perinuclear space. We will study the composition and function of tegument in these HSV assembly intermediates.

疱疹病毒是导致许多人类疾病的原因。所有的疱疹病毒都有一层蛋白质层，称为被膜，覆盖在病毒包膜的内表面。被膜和包膜的正确组装对于感染性病毒颗粒的产生以及因此对于疾病的进展是必不可少的。然而，很少有人知道被膜蛋白如何识别和结合到特定的细胞膜，也没有他们如何确保其组装成成熟的病毒粒子。本计划的第一部分将研究单纯疱疹病毒（HSV）蛋白VHS的生物化学，VHS是HSV外皮的一个组成部分。我们先前已经 在受感染的细胞中鉴定了几种生化上不同的VHS形式。我们现在将研究这些分子之间的关系，并确定它们对vhs组装到被膜中的重要性。接下来，我们将剖析vhs结合细胞内 膜。最后，我们已经成功地分离出初级包膜HSV颗粒从 核周空间我们将研究这些HSV组装中间体中被膜的组成和功能。