SORTING AND ASSEMBLY OF THE HSV-1 TEGUMENT PROTEIN VHS

疱疹病毒是导致大量人类疾病的原因。 所有疱疹病毒都有一层称为被膜的蛋白质层， 排列在病毒包膜的内表面 正确组装 被膜和被膜是产生传染性 病毒颗粒，从而导致疾病的进展。 然而，在这方面， 很少有人知道皮层蛋白如何识别和结合到特定的 细胞膜，也不知道它们如何确保组装成成熟的 病毒体。 这项建议将调查膜和皮层分类信息 存在于单纯疱疹病毒（HSV）被膜蛋白vhs内。 我们 将识别结合VHS的细胞质细胞器，并确定 这种关联在HSV生命周期中的作用。 序列 负责vhs/细胞器结合将被确定，我们将 确定VHS的哪些区域确保该蛋白质掺入到 成熟的病毒 从这项研究中获得的信息将有助于 能够干扰vhs组装到HSV中的试剂的设计 颗粒，降低这种严重的人类病原体的毒力。

The Herpes viruses are responsible for a large number of human diseases. All Herpes viruses possess a proteinaceous layer termed tegument which lines the inner surface of the viral envelope. Correct assembly of tegument and envelope is essential for the production of an infectious virus particle, and thus for the progression of disease. However, little is known of how tegument proteins recognize and bind to specific cellular membranes, nor how they ensure their assembly into the maturing virion. This proposal will investigate membrane and tegument sorting information present within the Herpes simplex virus (HSV) tegument protein vhs. We will identify the cytoplasmic organelle which binds vhs, and determine the role of this association in the HSV life cycle. Sequences responsible for vhs/organelle binding will be identified, and we will determine which regions of vhs ensure incorporation of this protein into the mature virus. Information obtained from this study will help in the design of agents able to interfere with vhs assembly into the HSV particle, reducing the virulence of this serious human pathogen.