STEROID-INITIATED EXOCYTOSIS IN SPERM
STEROID-INITIATED EXOCYTOSIS IN SPERM
批准号:
2888944
负责人:
STANLEY MEIZEL
金额:
$24.16万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-07-01 至 2002-06-30
关键词:
GABA receptor acrosome calcium flux chloride channels confocal scanning microscopy exocytosis fluorescent dye /probe hormone regulation /control mechanism human tissue immunoelectron microscopy immunoprecipitation membrane channels monoclonal antibody progesterone receptor expression reproductive system pharmacology sperm capacitation steroid hormone receptor transmission electron microscopy
中文摘要
描述(改编自《调查者摘要》):这项研究
三次修订的提案旨在了解分子步骤
参与人类精子顶体反应的启动。
卵丘卵泡细胞分泌的黄体酮
通过与精子相互作用被认为是体内顶体反应的启动者
膜受体触发钙离子的内流和氯离子的外流。在……里面
此外,黄体酮或3a-羟基黄体酮代谢物可结合或
激活一个独特的人精子GABAA样受体Cl-通道以及
与钙通道的受体结合。存在这样一种可能性:
黄体酮与一个受体/通道结合,然后激活另一个受体/通道
通过串扰实现接收器/通道。该应用程序包括一系列
关于受体、通道和离子相互作用的研究
黄体酮通过它启动人类精子顶体反应。这个
被提议的特定目标将首先决定类似于GABAA的精子
受体/氯离子通道参与孕酮介导的氯离子外流
使用将确定精子之间差异的药理学试剂
神经元GABAA样受体/氯离子通道。第二个目标是确定
黄体酮介导的胞浆氯离子在精子头部的哪个区域
通过类固醇受体/氯离子通道的外流开始于顶体
反应。第三个目标是使用可用的单抗来对抗
细胞内孕酮受体结构域和神经元GABAA样蛋白
受体/氯通道亚基用于识别和进一步表征人类
精子孕酮受体(S)。第四个目标是比较
孕酮立体异构体对氯离子和钙离子通量的测定
不同的孕激素受体可产生离子通量。第五个目标
是为了确定人类精子是否能将黄体酮转化为
3A-羟基代谢物可激活神经元GABAA样受体/Cl-
并能够启动人类顶体反应。第六个目标是
确定GABAA样受体/氯-是否介导氯-外流
通道控制孕酮介导的钙内流。最后,第七个目标
将使用免疫电子显微镜来确定
人孕酮受体(S)与人精子GABAA样受体/Cl-
高分辨率的通道。
英文摘要
DESCRIPTION (Adapted from the Investigator's Abstract): Research in this
three-times revised proposal is aimed at understanding the molecular steps
involved in the initiation of the human sperm acrosome reaction.
Progesterone secreted by follicular cells of the cumulus oophorus, is
regarded as an in vivo acrosome reaction initiator by interacting with sperm
membrane receptors to trigger an influx of Ca2+ and an efflux of Cl-. In
addition, progesterone or a 3a-OH progesterone metabolite may bind or
activate a unique human sperm GABAA-like receptor Cl- channel as well as
bind to a receptor for a Ca2+ channel. The possibility exists that
progesterone binds to one receptor/channel site and then activate the other
receptor/channel by cross-talk. The application includes a series of
studies concerned with the receptors, channels and ionic interactions
through which progesterone initiates the human sperm acrosome reaction. The
proposed specific aims will determine first whether a sperm GABAA-like
receptor/Cl- channel is involved in progesterone-mediated Cl- efflux by
using pharmacological agents which will determine differences between sperm
and neuronal GABAA-like receptor/Cl-channels. A second aim is to determine
which region of the sperm head the progesterone-mediated cytosolic Cl-
efflux through the steroid receptor/Cl-channel begins during acrosome
reaction. A third aim is to use available monoclonal antibodies against
domains of the intracellular progesterone receptor and neuronal GABAA-like
receptor/Cl- channel subunits to identify and further characterize the human
sperm progesterone receptor(s). A fourth aim is to compare the effect of
progesterone stereo isomers on Cl- and Ca2- flux to determine whether two
separate progesterone receptors operate to elicit ion fluxes. A fifth aim
is to determine whether human sperm can convert progesterone into an
3a-hydroxy metabolite known to activate neuronal GABAA-like receptor/Cl-
channels and able to initiate the human acrosome reaction. A sixth aim is
to determine whether Cl- efflux mediated by the GABAA-like receptor/Cl-
channel controls progesterone-mediated Ca2+ influx. Finally, a seventh aim
will use immunoelectron microscopy to determine the distribution of the
human progesterone receptor(s) and human sperm GABAA-like receptor/Cl-
channel at a high resolution level.
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Recognition of a human sperm surface protein involved in the progesterone-initiated acrosome reaction by antisera against an endomembrane progesterone binding protein from porcine liver.
通过抗猪肝内膜黄体酮结合蛋白的抗血清识别参与黄体酮引发的顶体反应的人类精子表面蛋白。
DOI:
10.1016/s0303-7207(99)00173-2
发表时间:
1999
期刊:
Molecular and cellular endocrinology
影响因子:
4.1
作者:
[Buddhikot,M, Falkenstein,E, Wehling,M, Meizel,S]
通讯作者:
Meizel,S
Progesterone-mediated efflux of cytosolic chloride during the human sperm acrosome reaction.
人精子顶体反应过程中黄体酮介导的胞质氯化物流出。
DOI:
10.1006/bbrc.1995.2197
发表时间:
1995
期刊:
Biochemical and biophysical research communications.
影响因子:
--
作者:
[Turner,KO, Meizel,S]
通讯作者:
Meizel,S
Progesterone initiation of the human sperm acrosome reaction: the obligatory increase in intracellular calcium is independent of the chloride requirement.
黄体酮引发人类精子顶体反应:细胞内钙的强制性增加与氯的需求无关。
DOI:
10.1016/0303-7207(94)90238-0
发表时间:
1994
期刊:
Molecular and cellular endocrinology
影响因子:
4.1
作者:
[Turner,KO, Garcia,MA, Meizel,S]
通讯作者:
Meizel,S
X-ray stereomicroscopy: high resolution 3-D imaging of human spermatozoa in aqueous suspension with natural contrast.
X 射线立体显微镜:对水悬浮液中的人类精子进行高分辨率 3D 成像,具有自然对比度。
DOI:
10.1111/j.1365-2818.1992.tb01514.x
发表时间:
1992
期刊:
Journal of microscopy
影响因子:
2
作者:
[LooJr,BW, Williams,S, Meizel,S, Rothman,SS]
通讯作者:
Rothman,SS
Chloride efflux during the progesterone-initiated human sperm acrosome reaction is inhibited by lavendustin A, a tyrosine kinase inhibitor.
黄体酮引发的人类精子顶体反应过程中的氯离子流出受到薰衣草丁 A(一种酪氨酸激酶抑制剂)的抑制。
DOI:
--
发表时间:
1996
期刊:
Journal of andrology
影响因子:
--
作者:
[Meizel,S, Turner,KO]
通讯作者:
Turner,KO
共 11 条
STEROID-INITIATED EXOCYTOSIS IN SPERM
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批准号:2025173
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项目类别:
-
资助金额:$23.22万
-
财政年份:1997
-
负责人:STANLEY MEIZEL
-
依托单位:
SPERM GLYCINE RECEPTOR AND ZONA-INITIATED EXOCYTOSIS
-
批准号:2883145
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项目类别:
-
资助金额:$22.63万
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财政年份:1997
-
负责人:STANLEY MEIZEL
-
依托单位:
SPERM GLYCINE RECEPTORS AND ZONA-INITIATED EXOCYTOSIS
-
批准号:6571377
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项目类别:
-
资助金额:$26.73万
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财政年份:1997
-
负责人:STANLEY MEIZEL
-
依托单位:
STEROID-INITIATED EXOCYTOSIS IN SPERM
-
批准号:2673550
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项目类别:
-
资助金额:$23.97万
-
财政年份:1997
-
负责人:STANLEY MEIZEL
-
依托单位:
SPERM GLYCINE RECEPTORS AND ZONA-INITIATED EXOCYTOSIS
-
批准号:6731181
-
项目类别:
-
资助金额:$26.73万
-
财政年份:1997
-
负责人:STANLEY MEIZEL
-
依托单位:
SPERM GLYCINE RECEPTORS AND ZONA-INITIATED EXOCYTOSIS
-
批准号:6843825
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项目类别:
-
资助金额:$26.73万
-
财政年份:1997
-
负责人:STANLEY MEIZEL
-
依托单位:
SPERM GLYCINE RECEPTOR AND ZONA-INITIATED EXOCYTOSIS
-
批准号:6164907
-
项目类别:
-
资助金额:$22.75万
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财政年份:1997
-
负责人:STANLEY MEIZEL
-
依托单位:
SPERM GLYCINE RECEPTOR AND ZONA-INITIATED EXOCYTOSIS
-
批准号:2668596
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项目类别:
-
资助金额:$21.44万
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财政年份:1997
-
负责人:STANLEY MEIZEL
-
依托单位:
SPERM GLYCINE RECEPTORS AND ZONA-INITIATED EXOCYTOSIS
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批准号:7007665
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项目类别:
-
资助金额:$26.1万
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财政年份:1997
-
负责人:STANLEY MEIZEL
-
依托单位:
SPERM GLYCINE RECEPTOR AND ZONA-INITIATED EXOCYTOSIS
-
批准号:2025701
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项目类别:
-
资助金额:$21.93万
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财政年份:1997
-
负责人:STANLEY MEIZEL
-
依托单位:
STEROID INITIATED EXOCYTOSIS IN HUMAN SPERM
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批准号:3323111
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项目类别:
-
资助金额:$15.13万
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财政年份:1990
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负责人:STANLEY MEIZEL
-
依托单位:
STEROID-INITIATED EXOCYTOSIS IN SPERM
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批准号:2198764
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项目类别:
-
资助金额:$17.36万
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财政年份:1990
-
负责人:STANLEY MEIZEL
-
依托单位:
STEROID-INITIATED EXOCYTOSIS IN HUMAN SPERM
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批准号:3323112
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项目类别:
-
资助金额:$15.9万
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财政年份:1990
-
负责人:STANLEY MEIZEL
-
依托单位:
STEROID-INITIATED EXOCYTOSIS IN HUMAN SPERM
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批准号:3323110
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项目类别:
-
资助金额:$11.51万
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财政年份:1990
-
负责人:STANLEY MEIZEL
-
依托单位:
STEROID-INITIATED EXOCYTOSIS IN HUMAN SPERM
-
批准号:3323113
-
项目类别:
-
资助金额:$16.65万
-
财政年份:1990
-
负责人:STANLEY MEIZEL
-
依托单位:
FERTILIZATION AND EARLY DEVELOPMENT
-
批准号:2609010
-
项目类别:
-
资助金额:$5.31万
-
财政年份:1978
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负责人:STANLEY MEIZEL
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依托单位:
FERTILIZATION AND EARLY DEVELOPMENT
-
批准号:2195120
-
项目类别:
-
资助金额:$5.02万
-
财政年份:1978
-
负责人:STANLEY MEIZEL
-
依托单位:
FERTILIZATION AND EARLY DEVELOPMENT
-
批准号:2195121
-
项目类别:
-
资助金额:$4.84万
-
财政年份:1978
-
负责人:STANLEY MEIZEL
-
依托单位:
FERTILIZATION AND EARLY DEVELOPMENT
-
批准号:2195119
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项目类别:
-
资助金额:$5.01万
-
财政年份:1978
-
负责人:STANLEY MEIZEL
-
依托单位:
FERTILIZATION AND EARLY DEVELOPMENT
-
批准号:3538968
-
项目类别:
-
资助金额:$3.13万
-
财政年份:1978
-
负责人:STANLEY MEIZEL
-
依托单位:
海外基金