REGULATION OF LENS FIBER CELL DIFFERENTIATION

晶状体纤维细胞分化的调节

• 批准号: 2888621
• 负责人: MELINDA K DUNCAN
• 金额: $ 25.38万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2888621
• 关键词: DNA binding protein; cataract; cell differentiation; crystallins; developmental genetics; fiber cell; gel mobility shift assay; gene expression; genetic promoter element; genetic regulation; genetic regulatory element; genetically modified animals; laboratory mouse; lens; molecular cloning; regulatory gene; transcription factor; transfection

DESCRIPTION: The underlying cause of cataracts can often be shown to involve defects in lens fiber cell differentiation. The proposal seeks to elucidate the mechanisms controlling the high level, lens-specific expression of the bB1 crystallin gene, a crystallin expressed exclusively in differentiating lens fiber cells. The aims of the proposal are to determine the role of the Prox-1 homeodomain protein in activation of the bB1 gene, to determine the role of Pax6-mediated repression of bB1 promoter in fiber cell differentiation, to identify other transcription factors that are involved in regulating expression of the bB1 gene and finally to identify sequences in the bB1 crystallin gene promoter that are required for appropriate spatial and temporal expression of bB1 crystallin during fiber cell differentiation.

描述：白内障的根本原因通常可以被证明是 涉及晶状体纤维细胞分化缺陷。该提案旨在 阐明控制高水平、晶状体特异性的机制 BB1晶体蛋白基因的表达，这是一种在 分化晶状体纤维细胞。该提案的目的是确定 Prox-1同源结构域蛋白在BB1基因激活中的作用 确定Pax6介导的BB1启动子在纤维细胞中的抑制作用 分化，以确定参与其中的其他转录因子 调控BB1基因的表达并最终鉴定序列 在BB1晶体蛋白基因启动子中需要适当的 BB1晶体蛋白在纤维细胞中的时空表达 差异化。