OPTICAL BIOPSY USING OPTICAL COHERENCE TOMOGRAPHY

使用光学相干断层扫描进行光学活检

• 批准号: 2769965
• 负责人: JAMES G FUJIMOTO
• 金额: $ 19.48万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-05 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2769965
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; biopsy; clinical biomedical equipment; diagnosis design /evaluation; diagnostic catheterization; disease /disorder model; early diagnosis; endoscopy; fiber optic microscopy; hamsters; histopathology; human tissue; imaging /visualization /scanning; laboratory rabbit; neoplasm /cancer diagnosis; preneoplastic state; tomography

The program is a multidisciplinary, collaborative effort which is a continuation of ongoing research involving investigators at the Massachusetts Institute of Technology and the Massachusetts General Hospital. The hypothesis of this proposal is that optical coherence tomography, a new optical diagnostic imaging technology for in situ imaging of tissue microstructure, can be developed and applied for "optical biopsy," the in situ diagnosis and monitoring of early neoplastic changes. The proposed program will consist of several components with complementary aims. Studies will be performed with existing OCT technology operating at image resolutions of 15-20 microns to examine neoplastic changes in tissue from various organ systems and establish correspondence with histopathology. Though some neoplastic changes such as glandular hypertropy are likely to occur at this resolution, some malignancies, such as cervical carcinoma, will likely require highter resolution to allow nuclear analysis. OCT technology will be developed in order to extend the limit of imaging resolution to the 3-4 micron scale to permit this cellular and subcellular level imaging. High resolution imaging studies and correspondence with histopathology will then be performed on selected tissue pathologies in order to evaluate the ability to visualize microstructure changes critical in identifying neoplastic transformation. We expect that imaging at 3-4 micron resolution should be an enabling step toward a wide range of diagnostic applications. In order to develop the foundation for in vivo studies, we will develop a forward scanning rigid endoscope and a forward scanning flexible catheter/endoscope. Together with a transverse scanning OCT catheter that we have already developed, we will perform in vivo imaging studies of normal tissue of the GI, urinary, reproductive, and respiratory tracts in a normal animal model. These studies will identify issues which must be addressed in vivo imaging studies and possible differences between in vitro and in vivo imaging. Finally we will perform in vivo imaging studies using the hamster cheek pouch carcinoma model to evaluate the ability of OCT to image premalignant and malignant changes in vivo. Taken together, these studies hold the promise of developing a new imaging diagnostic which could be a powerful tool in the diagnosis and prevention of malignancy.

该计划是一个多学科的，合作的努力，这是一个 继续进行涉及研究人员的正在进行的研究 马萨诸塞州理工学院和马萨诸塞州将军 医院 这个提议的假设是， 层析成像，一种新的光学诊断成像技术， 组织微观结构的成像，可以开发和应用于 “光学活检”，原位诊断和监测的早期 肿瘤性变化。 拟议的方案将包括几个 具有互补性的目标。 研究将在以下条件下进行： 现有的OCT技术在15-20的图像分辨率下操作 微米来检查来自各种器官的组织中的肿瘤变化 系统并与组织病理学建立对应关系。 虽然 某些肿瘤性改变，如腺体肥大， 在此分辨率下发生一些恶性肿瘤，例如宫颈癌 癌症，可能需要更高的分辨率，以允许核 分析. 将开发OCT技术，以扩展 成像分辨率的限制为3-4微米尺度，以允许这一点 细胞和亚细胞水平成像。 高分辨率成像 然后进行研究并与组织病理学进行通信 对选定的组织病理学，以评估能力， 可视化微结构变化在识别肿瘤中至关重要 转型 我们预计3-4微米分辨率的成像 应该是朝着广泛的诊断迈出的一步， 应用. 为了发展体内的基础 研究，我们将开发一种前向扫描刚性内窥镜和一种 前向扫描柔性导管/内窥镜。 连同 我们已经开发的横向扫描OCT导管， 将对胃肠道正常组织进行体内成像研究， 正常动物的泌尿、生殖和呼吸道 模型 这些研究将确定必须解决的问题 体内成像研究以及体外和 体内成像。 最后，我们将进行体内成像研究 用仓鼠颊囊癌模型评价 的OCT成像癌前病变和体内的恶性变化。 采取 这些研究共同为开发新的成像技术带来了希望 诊断，这可能是一个强大的工具， 预防恶性肿瘤。