Optical Biopsy sing Optical Coherence Tomography

描述(由申请人提供)： 这一提议的假设是光学相干层析成像(OCT)，一种新兴的生物医学！诊断成像技术用于组织微结构的原位成像，可开发并应用于“光学活组织检查”，实时、活体检测早期肿瘤变化。图像引导可以与切除活检相结合，以减少采样误差并提高灵敏度。该计划将新技术开发与生物医学和临床研究的应用相结合。具体目标是：1.发展超高分辨率OCT和光谱OCT技术，将目前10-15微米的分辨率提高到1-2微米的水平。这种图像分辨率的显著提高将显著提高OCT在建筑形态成像方面的性能，并将有助于识别早期肿瘤变化，如异型增生。光谱OCT成像将根据光谱特性改善组织的差异性，并有望提供细胞形态的评估。这些研究将利用我们团队开发的最先进的激光技术，在宽波长范围内实现超高分辨率成像，以优化图像穿透和分辨率。2.发展光学相干显微镜(OCM)技术，将OCT技术与共焦显微镜技术相结合。OCM能够实现细胞级别的分辨率，并且具有比共焦显微镜更大的图像穿透深度。它还可以在不需要共焦显微镜所需的高数值孔径的情况下进行成像，从而能够使用小型探头(如腹腔镜、内窥镜和针头)进行成像。3.研究正常动物和肿瘤进展模型的OCT成像。将在正常兔身上进行成像研究，以验证新的内窥镜成像技术。将使用仓鼠颊囊鳞状细胞癌模型和AOM大鼠结肠癌模型来成像癌症进展。4.研究胃肠道的超高分辨率OCT和光谱OCT成像。这一目标将涉及体外标本的成像和内窥镜成像研究。将探讨OCT成像在鉴别短段Barrett‘s食道、食管异型增生和食管腺癌、结肠腺瘤性息肉和增生性息肉中的可行性。这项计划是与退伍军人管理局波士顿医疗系统的研究人员进行的多学科合作。如果成功，这项研究将开发新的成像技术，应用于胃肠道内窥镜检查以及检测一系列早期肿瘤变化。

DESCRIPTION (provided by applicant): The hypothesis of this proposal is that optical coherence tomography (OCT), an emerging biomedical! diagnostic imaging technology for in situ imaging of tissue microstructure, can be developed and applied for "optical biopsy," the real time, in vivo detection of early neoplastic changes. Image guidance can be coupled with excisional biopsy to reduce sampling error and improve sensitivity. This program integrates new technology development with applications to biomedical and clinical studies. The specific aims are: 1. Develop ultrahigh resolution OCT and spectroscopic OCT technology, improving the current 10-15 um resolution to the 1-2 um level. This dramatic improvement in image resolution will significantly enhance the performance of OCT for imaging of architectural morphology and will facilitate the identification of early neoplastic changes such as dysplasia. Spectroscopic OCT imaging will improve differentiation of tissue based on spectroscopic properties and promises to provide assessment of cellular morphology. These studies will utilize state of the art laser technology developed in our group, enabling ultrahigh resolution imaging in broad wavelength regimes to optimize image penetration and resolution. 2. Develop new optical coherence microscopy (OCM) technology, which integrates OCT with confocal microscopy. OCM enables cellular level resolution and has image penetration depths greater than confocal microscopy. It also performs imaging without the need for high numerical apertures required in confocal microscopy, thus enabling imaging with small probes such as laparoscopes, endoscopes, and needles. 3. Investigate OCT imaging in normal animals and models of cancer progression. Imaging studies will be performed in the normal rabbit to validate new endoscopic imaging techniques. The hamster cheek pouch model of squamous cell carcinoma and the AOM rat model of colon cancer will be used to image cancer progression. 4. Investigate ultrahigh resolution OCT and spectroscopic OCT imaging of the GI tract. This aim will involve imaging of ex vivo specimens and endoscopic imaging studies. The feasibility of OCT imaging to differentiate short segment Barrett's esophagus, dysplasia and adenocarcinoma of the esophagus, and adenomatous vs. hyperplastic polyps in the colon will be explored. This program is a multidisciplinary collaborative effort with investigators at the VA Boston Healthcare system. If successful, this research will develop new imaging technology with applications in gastrointestinal endoscopy as well as the detection of a wide range of early neoplastic changes.