Optical Biopsy sing Optical Coherence Tomography

光学相干断层扫描光学活检

• 批准号: 7255707
• 负责人: JAMES G FUJIMOTO
• 金额: $ 22.81万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-05 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7255707
• 关键词: Adenocarcinoma; Animal Model; Animals; Arts; Azoxymethane; Barrett Esophagus; Biopsy; Boston; California; Cellular Morphology; Cheek structure; Clinical; Clinical Research; Collaborations; Colon; Colon Carcinoma; Confocal Microscopy; Coupled; Depth; Detection; Development; Devices; Diagnosis; Diagnostic Imaging; Disease; Doctor of Medicine; Doctor of Philosophy; Dysplasia; Early Diagnosis; Early identification; Endoscopes; Endoscopy; Esophageal Adenocarcinoma; Esophagus; Excision biopsy; Gastrointestinal Endoscopy; Gastrointestinal tract structure; Genus Cola; Gold; Hamsters; Healthcare Systems; Histology; Histopathology; Hyperplastic Polyp; Image; Imagery; Imaging Device; Imaging Techniques; Imaging technology; In Situ; Investigation; Laboratories; Laparoscopes; Lasers; Los Angeles; Lower Gastrointestinal Tract; Microscopy; Modeling; Morphology; Needles; Neoplasms; Nuclear; Optical Biopsy; Optical Coherence Tomography; Optics; Oryctolagus cuniculus; Pathology; Patients; Penetration; Performance; Property; Range; Rate; Rattus; Research; Research Personnel; Resolution; Sampling Errors; Specimen; Specimen Handling; Squamous cell carcinoma; Standards of Weights and Measures; Technology; Time; Tissue Differentiation; Tissues; Universities; Work; base; bioimaging; human subject; improved; in vivo; light scattering; multidisciplinary; neoplastic; new technology; optical imaging; programs; technology development; tomography; tumor progression

DESCRIPTION (provided by applicant): The hypothesis of this proposal is that optical coherence tomography (OCT), an emerging biomedical! diagnostic imaging technology for in situ imaging of tissue microstructure, can be developed and applied for "optical biopsy," the real time, in vivo detection of early neoplastic changes. Image guidance can be coupled with excisional biopsy to reduce sampling error and improve sensitivity. This program integrates new technology development with applications to biomedical and clinical studies. The specific aims are: 1. Develop ultrahigh resolution OCT and spectroscopic OCT technology, improving the current 10-15 um resolution to the 1-2 um level. This dramatic improvement in image resolution will significantly enhance the performance of OCT for imaging of architectural morphology and will facilitate the identification of early neoplastic changes such as dysplasia. Spectroscopic OCT imaging will improve differentiation of tissue based on spectroscopic properties and promises to provide assessment of cellular morphology. These studies will utilize state of the art laser technology developed in our group, enabling ultrahigh resolution imaging in broad wavelength regimes to optimize image penetration and resolution. 2. Develop new optical coherence microscopy (OCM) technology, which integrates OCT with confocal microscopy. OCM enables cellular level resolution and has image penetration depths greater than confocal microscopy. It also performs imaging without the need for high numerical apertures required in confocal microscopy, thus enabling imaging with small probes such as laparoscopes, endoscopes, and needles. 3. Investigate OCT imaging in normal animals and models of cancer progression. Imaging studies will be performed in the normal rabbit to validate new endoscopic imaging techniques. The hamster cheek pouch model of squamous cell carcinoma and the AOM rat model of colon cancer will be used to image cancer progression. 4. Investigate ultrahigh resolution OCT and spectroscopic OCT imaging of the GI tract. This aim will involve imaging of ex vivo specimens and endoscopic imaging studies. The feasibility of OCT imaging to differentiate short segment Barrett's esophagus, dysplasia and adenocarcinoma of the esophagus, and adenomatous vs. hyperplastic polyps in the colon will be explored. This program is a multidisciplinary collaborative effort with investigators at the VA Boston Healthcare system. If successful, this research will develop new imaging technology with applications in gastrointestinal endoscopy as well as the detection of a wide range of early neoplastic changes.

描述（由申请人提供）：