Optical Biopsy sing Optical Coherence Tomography

描述（由申请人提供）： 该提案的假设是，光学相干断层扫描（OCT），一种新兴的生物医学！诊断成像技术用于组织微观结构的原位成像，可开发和应用于“光学活检”，真实的时间，在体内检测早期肿瘤变化。图像引导可以与切除活检相结合，以减少采样误差并提高灵敏度。该计划将新技术开发与生物医学和临床研究应用相结合。具体目标是：1.发展高分辨率OCT和光谱OCT技术，将目前10-15 μ m分辨率提高到1-2 μ m水平。图像分辨率的显著提高将显著增强OCT用于建筑形态成像的性能，并将有助于识别早期肿瘤变化，如发育异常。光谱OCT成像将改善基于光谱特性的组织区分，并有望提供细胞形态的评估。这些研究将利用我们小组开发的最先进的激光技术，使宽波长范围内的高分辨率成像能够优化图像穿透力和分辨率。2.开发新的光学相干显微镜（OCM）技术，将OCT与共焦显微镜相结合。OCM能够实现细胞水平的分辨率，并且具有比共聚焦显微镜更大的图像穿透深度。它还可以在不需要共焦显微镜所需的高数值孔径的情况下进行成像，从而能够使用腹腔镜，内窥镜和针头等小型探头进行成像。3.研究正常动物和癌症进展模型中的OCT成像。将在正常家兔中进行成像研究，以验证新的内窥镜成像技术。鳞状细胞癌的仓鼠颊囊模型和结肠癌的AOM大鼠模型将用于对癌症进展进行成像。4.研究胃肠道的高分辨率OCT和光谱OCT成像。这一目标将涉及离体标本的成像和内窥镜成像研究。将探讨OCT成像区分短节段Barrett食管、食管异型增生和腺癌以及结肠腺瘤性息肉与增生性息肉的可行性。该计划是与VA波士顿医疗保健系统的研究人员进行的多学科合作。如果成功，这项研究将开发新的成像技术，应用于胃肠道内窥镜检查以及检测广泛的早期肿瘤变化。

DESCRIPTION (provided by applicant): The hypothesis of this proposal is that optical coherence tomography (OCT), an emerging biomedical! diagnostic imaging technology for in situ imaging of tissue microstructure, can be developed and applied for "optical biopsy," the real time, in vivo detection of early neoplastic changes. Image guidance can be coupled with excisional biopsy to reduce sampling error and improve sensitivity. This program integrates new technology development with applications to biomedical and clinical studies. The specific aims are: 1. Develop ultrahigh resolution OCT and spectroscopic OCT technology, improving the current 10-15 um resolution to the 1-2 um level. This dramatic improvement in image resolution will significantly enhance the performance of OCT for imaging of architectural morphology and will facilitate the identification of early neoplastic changes such as dysplasia. Spectroscopic OCT imaging will improve differentiation of tissue based on spectroscopic properties and promises to provide assessment of cellular morphology. These studies will utilize state of the art laser technology developed in our group, enabling ultrahigh resolution imaging in broad wavelength regimes to optimize image penetration and resolution. 2. Develop new optical coherence microscopy (OCM) technology, which integrates OCT with confocal microscopy. OCM enables cellular level resolution and has image penetration depths greater than confocal microscopy. It also performs imaging without the need for high numerical apertures required in confocal microscopy, thus enabling imaging with small probes such as laparoscopes, endoscopes, and needles. 3. Investigate OCT imaging in normal animals and models of cancer progression. Imaging studies will be performed in the normal rabbit to validate new endoscopic imaging techniques. The hamster cheek pouch model of squamous cell carcinoma and the AOM rat model of colon cancer will be used to image cancer progression. 4. Investigate ultrahigh resolution OCT and spectroscopic OCT imaging of the GI tract. This aim will involve imaging of ex vivo specimens and endoscopic imaging studies. The feasibility of OCT imaging to differentiate short segment Barrett's esophagus, dysplasia and adenocarcinoma of the esophagus, and adenomatous vs. hyperplastic polyps in the colon will be explored. This program is a multidisciplinary collaborative effort with investigators at the VA Boston Healthcare system. If successful, this research will develop new imaging technology with applications in gastrointestinal endoscopy as well as the detection of a wide range of early neoplastic changes.