Optical Biopsy Using Optical Coherence Tomography

使用光学相干断层扫描进行光学活检

基本信息

批准号：
7667472
负责人：
JAMES G FUJIMOTO
金额：
$ 27.14万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-05 至 2013-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7667472
关键词：
Adenocarcinoma Arts Barrett Esophagus Biomedical Research Biopsy Boston Breast Cellular Structures Classification Clinic Clinical Clinical Research Colon Colon Adenocarcinoma Computer Assisted Confocal Microscopy Crohn&apos s disease Data Data Set Detection Development Device Designs Devices Diagnostic Diagnostic Imaging Doctor of Philosophy Dysplasia Early Diagnosis Endoscopy Epithelial Esophageal Adenocarcinoma Esophagus Excision Excision biopsy Face Gastrointestinal Endoscopy Gastrointestinal tract structure Generations Gold Healthcare Systems Histology Histopathology Human Image Imagery Imaging Device Imaging Techniques Imaging technology In Situ Inflammatory Bowel Diseases Institutes Israel Laboratories Lasers Lesion Libraries Lower Gastrointestinal Tract Malignant Neoplasms Massachusetts Measurement Medical center Methodology Methods Microscopy Morphology Neoplasms Optical Biopsy Optical Coherence Tomography Optics Pathology Performance Pilot Projects Process Research Research Personnel Resolution Sampling Errors Scanning Sensitivity and Specificity Source Specimen Specimen Handling Speed Structure System Techniques Technology Three-Dimensional Imaging Time Tissues Ulcerative Colitis Upper digestive tract structure adenoma bioimaging cellular imaging clinical application design diagnosis standard image processing imaging probe improved in vivo light scattering medical schools neoplastic new technology optical imaging programs technique development technology development virtual

项目摘要

DESCRIPTION (provided by applicant): Optical coherence tomography (OCT) is an emerging imaging technology which performs high resolution, cross-sectional and three-dimensional (3D) imaging of tissue morphology in situ and in real time. Optical coherence microscopy (OCM) combines OCT with confocal microscopy to achieve cellular level resolution with an extended imaging depth. The hypothesis is that OCT and OCM can function as a type of "optical biopsy" to visualize early neoplastic changes in vivo without excision and processing of specimens. If successful, these imaging technologies could guide biopsy to reduce sampling error and improve sensitivity. This program vertically integrates technology development, biomedical research and clinical studies in a collaborative effort between investigators at the Massachusetts Institute of Technology, Beth Israel Deaconess Medical Center, Boston VA Healthcare System, and Harvard Medical School. The specific aims are: 1. Develop Ultrahigh Resolution 3D-OCT Imaging Technology. 3D-OCT will enable new visualization techniques such as generation of arbitrary cross-sectional images, projection views similar to microscopy, improved quantitative measurements of morphology and virtual manipulation of tissue for visualizing structure. We propose to develop new technology for clinical endoscopic 3D-OCT to achieve imaging speeds of 500,000 axial scans / second with <5 ¿m axial image resolution, factors of ~100x faster speed and ~2-3x finer resolution than standard OCT. 2. Develop Endoscopic Optical Coherence Microscopy (OCM) for Cellular Level Imaging. Developing techniques for cellular resolution endoscopy is a longstanding challenge in biomedical imaging and promises to improve early diagnosis of cancer. Optical coherence microscopy (OCM) is an in vivo cellular imaging technique which combines OCT with confocal microscopy. We propose to develop new OCM technology to enable cellular level endoscopic imaging. 3. Ex Vivo Imaging Studies Using 3D-OCT and OCM. Ex vivo imaging is a powerful methodology to establish correlations between OCT and OCM diagnostic features and histopathology. We will investigate upper and lower Gl tract pathologies including Barrett's esophagus, dysplasia, and adenocarcinoma, as well as dysplasia and adenocarcinoma in inflammatory bowel disease. We will also investigate breast malignancies. These studies will establish correspondence with histology, develop new visualization methods, validate computer assisted tissue classification techniques, and assess pilot applications in new pathologies. 4. Clinical Endoscopic Imaging of the Upper and Lower Gastrointestinal Tract. The hypothesis of this aim is that 3D-OCT and OCM imaging in the gastrointestinal tract can identify neoplastic changes. We will investigate Barrett's esophagus, dysplasia, and adenocarcinoma in the upper GI tract and dysplasia and adenocarcinoma of the colon in inflammatory bowel diseases in the lower GI tract. If successful, the proposed research will develop and demonstrate new imaging technology which will augment gastrointestinal endoscopy and open the door for improved detection of a wide range of neoplasias.

描述（由适用提供）：光学相干断层扫描（OCT）是一种新兴的成像技术，可在原位和实时进行组织形态的高分辨率，横截面和三维（3D）成像。光学相干显微镜（OCM）将OCT与共聚焦显微镜结合在一起，以实现细胞水平分辨率，并具有扩展的成像深度。假设是OCT和OCM可以用作“光学活检”的一种类型，可在不切除和处理试样的情况下可视化体内的早期肿瘤变化。如果成功，这些成像技术可以指导活检以减少采样误差并提高灵敏度。该计划将马萨诸塞州理工学院，贝丝·以色列执事医疗中心，波士顿VA Healthcare System和哈佛医学院的研究人员在研究人员之间的合作工作中垂直整合了技术开发，生物医学研究和临床研究。具体目的是：1。开发超高分辨率3D-OCT成像技术。 3D-OCT将启用新的可视化技术，例如，任意横截面图像的产生，类似于显微镜的投影视图，改进的形态学定量测量以及对组织的虚拟操纵以可视化结构。我们建议为临床内窥镜3D-OCT开发新技术，以实现500,000轴向扫描 /秒的成像速度，其轴向图像分辨率<5»轴向图像分辨率，速度更快〜100倍，比标准OCT分辨率〜2-3x。 2。开发用于细胞水平成像的内窥镜光学相干显微镜（OCM）。在生物医学成像中开发了内窥镜分辨率的技术是一项长期挑战，并有望改善癌症的早期诊断。光学相干显微镜（OCM）是一种体内细胞成像技术，将OCT与共聚焦显微镜结合在一起。我们建议开发新的OCM技术，以实现细胞水平的内窥镜成像。 3。使用3D-OCT和OCM进行体内成像研究。离体成像是建立OCT和OCM诊断特征与组织病理学之间建立相关性的有力方法。我们将研究炎症性肠病中的上和下GL道病理学，包括Barrett的食管，发育不良和腺癌，以及发育不良和腺癌的发育不良和腺癌。我们还将调查乳房恶性肿瘤。这些研究将与组织学，开发新的可视化方法，验证计算机辅助组织分类技术以及在新病理中的评估试验应用中建立对应关系。 4。上和下胃肠道的临床内窥镜成像。该目标的假设是胃肠道中的3D-OCT和OCM成像可以鉴定肿瘤变化。我们将研究Barrett的食管，发育异常和腺癌在胃肠道上的胃肠道和发育不良和腺癌的炎症性肠道疾病中的炎症性疾病。如果成功的话，拟议的研究将开发并展示新的成像技术，该技术将增强胃肠道内窥镜检查，并为改善对广泛的新层次的检测打开大门。